Table 3 Comparison of LOD, LOQ, RSD and percentage recovery of the developed method with the literature methods
Compound | LOD (µg/L) | LOQ (µg/L) | RSD (%) | Recovery (%) | Method | References |
|---|---|---|---|---|---|---|
Caffeine | 3610 | 10,940 | 1.7 | 95 ± 7 | HPLC–DAD–MS | [4] |
Theobromine | 30 | 100 | 6.5 | 97 ± 3 | ||
Trigonelline | 3770 | 11,420 | 1.0 | 102 ± 2 | ||
Caffeine | 80 | – | 0.20–6.2 | 99 ± 3 | HPLC–UV | [9] |
Theobromine | 140 | 2.8–9.1 | 103 ± 6 | |||
Trigonelline | 260 | 1.1–5.0 | 99 ± 5 | |||
Caffeine | 11.9 | 39.6 | 2.1–4.7 | 97 ± 4 | LC–MS | [12] |
Theobromine | – | – | – | – | ||
Trigonelline | 36.4 | 121.5 | 3.2–3.5 | 140 ± 16 | ||
Caffeine | 2 | 40 | 3 | 99 ± 2 | Fluorescence | This study |
Theobromine | 6 | 20 | 3 | – | ||
Trigonelline | 7 | 20 | 4 | 99 ± 2 |