New fluorescence spectroscopic method for the simultaneous determination of alkaloids in aqueous extract of green coffee beans

Table 2 Recovery results of caffeine and trigonelline by the developed fluorescence method

Origin of green coffee beans sample	Type of alkaloid in the sample	Amount of alkaloid in the sample before spiking (mg/L)	Amount of alkaloid added (mg/L)	Amount of alkaloid found after spiking (mg/L)	Recovery (%) (n = 3)
Abosto (Sidama)	Caffeine	67.1	2	69.1	102 ± 3
Abosto (Sidama)	Trigonelline	73.3	2	75.2	96.0 ± 3
Gedeo zone	Caffeine	59.3	2	61.2	94.0 ± 2
Gedeo zone	Trigonelline	124	2	126	99.0 ± 2
Wendogenet (Sidama)	Caffiene	73.2	2	75.2	101 ± 2
Wendogenet (Sidama)	Trigonelline	93.2	2	95.2	102 ± 2

ISSN: 2661-801X