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Table 1 Inhibitory effect of N-[{(2-phenyl)methylidene]-2-(4-ethyl-5-(pyridine-2-yl)-4H-1,2,4-triazole-3-yl)sulfanyl}acetohydrazide (ZE-4b), N-[{(2-phenyl)methylidene]-2-(4-(fluorophenyl-5-(pyridine-2-yl)-4H-1,2,4-triazole-3yl)sulfanyl}acetohydrazide (ZE-4c), N-[{(4-methylphenyl)sulfonyl}]-2-(4-cyclohexyl-5-(pyridine-2-yl)-4H-1,2,4-triazole-3-yl)sulfanyl}acetohydrazide (ZE-5a) and N-[{(4-methylphenyl) sulfonyl}-2-(4-ethyl-5-(pyridine-2-yl)-4H-1,2,4-triazole-3yl)sulfanyl}acetohydrazide (ZE-5b) on arachidonic acid (AA), adenosine diphosphate (ADP) and collagen induced platelet aggregation

From: Synthesis, characterization, molecular docking evaluation, antiplatelet and anticoagulant actions of 1,2,4 triazole hydrazone and sulphonamide novel derivatives

Test sample Agonists % inhibition of platelet aggregation IC50 (µM)
1 µM 3 µM 10 µM 30 µM 100 µM 300 µM 1000 µM
ZE-4b AA 4.4 ± 0.09 8.8 ± 0.09 30.3 ± 0.06 41.2 ± 0.23 63.2 ± 0.06 78 ± 0.14 89.5 ± 0.23 40.1
ADP 0.1 ± 0.03 1.0 ± 0.03 3.6 ± 0.03 9.6 ± 0.06 18.2 ± 0.12 39.4 ± 0.17 54.7 ± 0.18 785
Collagen 27.1 ± 0.40 39.2 ± 0.06 49.7 ± 0.11 63.7 ± 0.23 85.7 ± 0.06 43.8 ± 0.35 20.5 ± 0.35 10.01
ZE-4c AA 7.9 ± 0.15 15.4 ± 0.20 29 ± 0.21 43 ± 0.18 59 ± 0.03 75 ± 0.10 86.4 ± 0.44 55.3
ADP 0.1 ± 0.03 2.7 ± 0.06 9.6 ± 0.15 22.5 ± 0.06 32 ± 0.12 39.7 ± 0.23 52.8 ± 0.12 850.4
Collagen 33.5 ± 0.81 42.2 ± 0.24 50 ± 0.32 58.4 ± 0.32 68.4 ± 0.24 80.9 ± 0.26 85.9 ± 0.18 10
ZE-5a AA 4.0 ± 0.12 7.9 ± 0.06 23.7 ± 0.15 39.5 ± 0.21 47.4 ± 0.12 68 ± 0.35 72.8 ± 0.59 121.6
ADP 0.1 ± 0.09 1.8 ± 0.06 12.2 ± 0.12 24.3 ± 0.09 28.5 ± 0.12 36.3 ± 0.18 50.9 ± 0.17 956.8
Collagen 23.3 ± 0.11 37.8 ± 0.49 43.3 ± 0.17 49.5 ± 0.23 67.6 ± 0.58 72.9 ± 0.46 81.4 ± 0.11 30.1
ZE-5b AA 8.8 ± 0.09 11.4 ± 0.27 25 ± 0.21 30.7 ± 0.58 52.2 ± 0.40 68.4 ± 0.40 79 ± 0.60 99.9
ADP 1 ± 0.03 3.6 ± 0.06 8.7 ± 0.17 22.5 ± 0.06 37.1 ± 0.14 44.9 ± 0.03 61.2 ± 0.17 519
Collagen 21.6 ± 0.35 23.1 ± 0.41 43.8 ± 0.65 51.8 ± 0.43 67.8 ± 0.52 78.6 ± 0.31 91.1 ± 0.67 29.97
Aspirin AA 27.2 ± 0.18 36 ± 0.09 50.1 ± 0.16 59.7 ± 0.09 100 ± 0 100 ± 0 100 ± 0 10.01
ADP 3.6 ± 0.07 6.2 ± 0.09 19.1 ± 0.07 25 ± 0.06 32.8 ± 0.10 49.8 ± 0.12 56.9 ± 0.18 308.4
Collagen 37.2 ± 0.14 48.7 ± 0.14 57.7 ± 0.20 68.6 ± 0.29 71 ± 0.23 78.6 ± 0.23 98.1 ± 0.11 3.2
  1. Values are shown as mean of % platelet aggregation inhibition ± SEM, n = 3–4