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Table 4 Linearity and regression equation of the flavonoid and phenolic compounds

From: Assessment and comparison of phytochemical constituents and biological activities of bitter bean (Parkia speciosa Hassk.) collected from different locations in Malaysia

Compounds UV (λmax) Rt (min) Linear regresion R2 LOD (µg mL−1) LOQ (µg mL−1)
Quercetin 355 10.2 y = 92.846x + 37.26 0.9991 0.91 3.02
Rutin 260 4.8 y = 86.437x + 22.71 0.9984 1.20 3.98
Kaempferol 275 18.7 y = 146.209x + 30.61 0.9947 0.67 2.24
Catechin 280 3.9 y = 452.017x + 62.19 0.9996 0.16 0.53
Luteolin 275 14.5 y = 265.733x + 46.52 0.9993 0.30 0.99
Myricetin 275 12.8 y = 109.357x + 59.34 0.9957 0.83 2.82
Gallic acid 280 2.6 y = 864.620x-114.17 0.9928 0.05 0.18
Ferulic acid 320 6.4 y = 640.052x + 88.14 0.9991 0.12 0.39
Caffeic acid 280 3.8 y = 261.55x + 56.20 0.9970 0.28 0.93
trans-Cinnamic acid 280 4.7 y = 173.062x + 44.91 0.9994 0.58 1.91
p-coumaric acid 320 11.1 y = 243.526x + 84.28 0.9961 0.34 1.13
  1. R t retention time, y peak area, x concentration of standard (µg mL−1), R2 correlation coefficient for six data point in the calibration carve (n = 3), LOD limit of detection, LOQ limit of quantification