Assessment and comparison of phytochemical constituents and biological activities of bitter bean (Parkia speciosa Hassk.) collected from different locations in Malaysia

Table 4 Linearity and regression equation of the flavonoid and phenolic compounds

Compounds	UV (λ_max)	R_t (min)	Linear regresion	R²	LOD (µg mL⁻¹)	LOQ (µg mL⁻¹)
Quercetin	355	10.2	y = 92.846x + 37.26	0.9991	0.91	3.02
Rutin	260	4.8	y = 86.437x + 22.71	0.9984	1.20	3.98
Kaempferol	275	18.7	y = 146.209x + 30.61	0.9947	0.67	2.24
Catechin	280	3.9	y = 452.017x + 62.19	0.9996	0.16	0.53
Luteolin	275	14.5	y = 265.733x + 46.52	0.9993	0.30	0.99
Myricetin	275	12.8	y = 109.357x + 59.34	0.9957	0.83	2.82
Gallic acid	280	2.6	y = 864.620x-114.17	0.9928	0.05	0.18
Ferulic acid	320	6.4	y = 640.052x + 88.14	0.9991	0.12	0.39
Caffeic acid	280	3.8	y = 261.55x + 56.20	0.9970	0.28	0.93
trans-Cinnamic acid	280	4.7	y = 173.062x + 44.91	0.9994	0.58	1.91
p-coumaric acid	320	11.1	y = 243.526x + 84.28	0.9961	0.34	1.13

R_t retention time, y peak area, x concentration of standard (µg mL⁻¹), R² correlation coefficient for six data point in the calibration carve (n = 3), LOD limit of detection, LOQ limit of quantification

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