Table 7 System suitability testing parameters of the developed methods
From: Determination of flutamide and two major metabolites using HPLC–DAD and HPTLC methods
Parameters | HPTLC method | HPLC method | ||||
|---|---|---|---|---|---|---|
Flu-1 | Flutamide | Flu-3 | Flu-3 | Flu-1 | Flutamide | |
Rf (for HPTLC) or Rt (for HPLC) | 0.48 ± 0.01 | 0.6 ± 0.01 | 0.74 ± 0.02 | 4.01 ± 0.06 | 6.00 ± 0.01 | 8.96 ± 0.02 |
Peak symmetry | 1.00 | 1.00 | 0.94 | 1.30 | 1.25 | 1.10 |
Selectivity (α) | ||||||
Plasma | 9.14 | 1.23 | 1.24 | 3.54 | 1.71 | 1.62 |
Urine | 9.8 | 1.71 | ||||
Resolution (Rs) | ||||||
Plasma | 10.18 | 2.00 | 2.24 | 4.60 | 5.24 | 5.60 |
Urine | 9.58 | 2.77 | ||||
Capacity factor (α) | 1.04 | 0.64 | 0.32 | 2.34 | 4.00 | 6.48 |
Number of theoretical plates (N) | 3059.22 | 2515.28 | 3698.29 | |||
Height equivalent to theoretical plate (H) (in cm) | 0.0049 | 0.0060 | 0.0041 | |||