Table 2 Intra and inter assay precision and accuracy
From: Determination of flutamide and two major metabolites using HPLC–DAD and HPTLC methods
Component | Concentration (µg/band)a | Intraday | Interday | ||||
|---|---|---|---|---|---|---|---|
Recovery % | Bias %b | RSD% | Recovery % | Bias% | RSD% | ||
a. For HPTLC method | |||||||
In plasma | |||||||
Flu | 0.3 (LLOQ) | 93.98 | − 6.02 | 6.526 | 93.97 | − 6.03 | 8.169 |
0.5 (LQC) | 97.88 | − 2.12 | 3.183 | 98.79 | − 1.21 | 4.33 | |
1.6 (MQC) | 103.54 | 3.54 | 3.063 | 105.353 | 5.53 | 4.538 | |
2.5 (HQC) | 98.61 | − 1.39 | 2.42 | 104.63 | 4.63 | 5.97 | |
Flu-1 | 0.3 (LLOQ) | 103.69 | 3.69 | 6.191 | 106.49 | 6.49 | 7.954 |
0.5 (LQC) | 103.61 | 33.61 | 3.703 | 104.95 | 4.95 | 4.085 | |
1.6 (MQC) | 98.11 | − 1.89 | 4 | 97.99 | − 2.01 | 4.614 | |
2.5 (HQC) | 97.87 | − 2.13 | 3.053 | 96.84 | − 3.16 | 4.917 | |
Flu-3 | 0.3 (LLOQ) | 105.34 | 5.34 | 7.934 | 96.19 | − 3.81 | 9.088 |
0.5 (LQC) | 102.36 | 2.36 | 4.129 | 104.76 | 4.76 | 5.688 | |
1.6 (MQC) | 102.93 | 2.93 | 4.61 | 104.38 | 4.338 | 5.699 | |
2.5 (HQC) | 97.338 | − 2.62 | 4.645 | 98.61 | 1.39 | 6.788 | |
In urine | |||||||
Flu | 0.3 (LLOQ) | 101.13 | 1.13 | 4.978 | 104.84 | 4.84 | 8.015 |
0.5 (LQC) | 95.78 | − 4.22 | 3.193 | 101.73 | 1.73 | 7.778 | |
1.6 (MQC) | 98.75 | − 1.25 | 2.051 | 99.82 | − 0.18 | 2.797 | |
3 (HQC) | 98.31 | − 1.69 | 2.446 | 98.99 | − 1.01 | 3.597 | |
Flu-1 | 0.3 (LLOQ) | 97.14 | − 2.86 | 7.44 | 107.87 | 7.87 | 7.694 |
0.5 (LQC) | 98.93 | − 1.07 | 3.282 | 98.2 | − 1.8 | 4.157 | |
1.6 (MQC) | 103.29 | 3.29 | 2.679 | 98.06 | − 1.94 | 2.985 | |
2 (HQC) | 99.23 | − 0.77 | 2.771 | 97.89 | − 2.11 | 4.175 | |
Flu-3 | 0.3 (LLOQ) | 103.82 | 3.82 | 8.481 | 106.31 | 6.31 | 9.175 |
0.5 (LQC) | 102.7 | 2.7 | 3.223 | 104.1 | 4.1 | 3.801 | |
1.6 (MQC) | 97.82 | − 2.18 | 2.685 | 101.96 | 1.96 | 3.698 | |
3 (HQC) | 98.67 | − 1.33 | 2.428 | 97.94 | − 2.06 | 5.032 | |
Component | Concentration (µg/band)a | Intraday | Interday | ||||
|---|---|---|---|---|---|---|---|
Recovery % | Bias %b | RSD% | Recovery % | Bias % | RSD% | ||
b. For HPLC method | |||||||
In plasma | |||||||
Flu | 2 (LLOQ) | 102.04 | 2.04 | 4.389 | 109.41 | 9.41 | 8.903 |
5 (LQC) | 97.98 | − 2.02 | 3.368 | 102.63 | 2.63 | 5.329 | |
20 (MQC) | 98.97 | − 1.03 | 2.319 | 97.82 | − 2.18 | 3.572 | |
45 (HQC) | 98.56 | − 1.44 | 3.404 | 99.28 | − 0.72 | 4.138 | |
Flu-1 | 2 (LLOQ) | 96 | − 4 | 5.657 | 104.32 | 4.32 | 10.386 |
5 (LQC) | 98.29 | − 1.71 | 3.282 | 98.57 | − 1.43 | 5.77 | |
20 (MQC) | 98.98 | − 1.02 | 1.982 | 99.83 | − 0.17 | 2.317 | |
45 (HQC) | 97.52 | − 2.48 | 2.271 | 98.86 | − 1.14 | 2.321 | |
Flu-3 | 15 (LLOQ) | 100.98 | 0.98 | 3.894 | 104.4 | 4.4 | 6.945 |
30 (LQC) | 98.6 | − 1.4 | 3.075 | 101.44 | 1.44 | 5.8 | |
100 (MQC) | 100.54 | 0.54 | 3.494 | 103.25 | 3.25 | 6.2 | |
170 (HQC) | 98.54 | − 1.46 | 3.92 | 95.79 | − 4.21 | 6.116 | |
In urine | |||||||
Flu | 2 (LLOQ) | 101.91 | 1.91 | 4.298 | 98.84 | − 1.16 | 7.831 |
5 (LQC) | 97.71 | − 2.29 | 4.01 | 97.27 | − 2.73 | 4.968 | |
20 (MQC) | 103.16 | 3.16 | 3.415 | 101.29 | 1.29 | 6.772 | |
45 (HQC) | 101.59 | 1.59 | 3.353 | 104.4 | 4.4 | 4.92 | |
Flu-1 | 2 (LLOQ) | 98.82 | − 1.18 | 3.734 | 96.8 | − 3.2 | 8.483 |
5 (LQC) | 99.57 | − 0.43 | 2.051 | 97.99 | − 2.01 | 3.594 | |
20 (MQC) | 99.29 | − 0.71 | 2.144 | 104.29 | 4.29 | 4.705 | |
45 (HQC) | 101.19 | 1.19 | 1.652 | 105.15 | 5.15 | 4.46 | |
Flu-3 | 15 (LLOQ) | 102.31 | 2.31 | 5.014 | 103.94 | 3.94 | 5.014 |
30 (LQC) | 99.17 | − 0.83 | 2.228 | 102.31 | 2.31 | 4.49 | |
100(MQC) | 100.55 | 0.55 | 1.79 | 102.45 | 2.45 | 3.994 | |
170 (HQC) | 100.32 | 0.32 | 1.335 | 101.37 | 1.37 | 2.533 | |