Table 1 Assay and method validation parameters for the determination of flutamide and its metabolites in plasma and urine samples by the proposed methods

Range^a

0.30–3.0

0.30–3.0

0.30–3.0

0.3–4.0

0.3–2.5

0.3–4.0

2–50

2–50

15–200

2–50

2–50

15–200

Slope

− 655.53^b

5414.98^c

− 1659.60^c

12446.00^d

− 455.09^c

2573.60^d

− 261.41^c

41861.50^d

− 2182.80^c

13125.00^d

− 139.66^c

1829.70^d

60.0650

41.5830

5.8755

51.8810

37.2350

6.3446

Intercept

665.50

3039.00

1240.80

1385.10

3066.40

305.60

− 13.2490

− 2.8848

25.9910

− 21.3530

− 0.8850

35.0270

Correlation (r)

0.9998

0.9998

0.9992

0.9997

0.9994

0.9996

0.9999

0.9997

0.9999

0.9998

0.9999

0.9997

LLOQ

0.30

0.30

0.30

0.30

0.30

0.30

2.0

2.0

15.0

2.0

2.0

15.0