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Fig. 2 | Chemistry Central Journal

Fig. 2

From: Investigating and characterizing the binding activity of the immobilized calmodulin to calmodulin-dependent protein kinase I binding domain with atomic force microscopy

Fig. 2

The Scheme for CaM patterns fabrication. a The OTSpd disk patterns were fabricated by local oxidation lithography. b MUTMS was cross-linked onto the OTSpd patterns, converting the OTSpd patterns into thiol-terminated surfaces. c Substrate was then incubated into HgCl2 solution to form Hg-SH coupling. d Cysteine-mutated CaM was immobilized on the chemical patterns via cysteine-Hg-SH coupling. e Structural model of substrate corresponding to part (d)

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