Table 5 Robustness of the developed analytical method
Chromatographic condition | Assay % | tR (min) | Theoretical plates | Symmetry |
|---|---|---|---|---|
Flow rate (0.9 mL min−1) | 98.0 | 4.747 | 8247 | 0.90 |
Flow rate (1 mL min−1) | 99.7 | 4.279 | 7910 | 0.90 |
Flow rate (1.1 mL min−1) | 102.7 | 3.868 | 7438 | 0.91 |
Methanol:buffer (12:88) | 98.3 | 5.109 | 8218 | 0.93 |
Methanol:buffer (15:85) | 99.7 | 4.270 | 7878 | 0.91 |
Methanol:buffer (18:82) | 97.5 | 3.040 | 6898 | 0.90 |
Buffer (pH 3.0) | 104.3 | 4.270 | 8218 | 0.93 |
Buffer (pH 3.5) | 100.1 | 4.276 | 8360 | 0.95 |
Buffer (pH 4.0) | 98.5 | 4.262 | 8076 | 0.90 |