Optimization of enzymatic esterification of dihydrocaffeic acid with hexanol in ionic liquid using response surface methodology

Table 1 ANOVA and regression coefficient of the first and second degree polynomial regression model

Sl. no.	Terms	P value^a	F ratio	Regression coefficient	Response
1	Constant		–	β₀	−300.110
2	X₁	0.000	29.06	β₁	18.654
3	X₂	0.007	9.84	β₂	3.831
4	X₃	0.002	14.65	β₃	1.182
5	X₄	0.000	22.91	β₄	8.425
6	\( {\text{X}}_{1}^{2} \)	0.000	83.20	β₁₂	−1.409
7	\( {\text{X}}_{2}^{2} \)	0.007	9.64	β₂₂	−0.052
8	\( {\text{X}}_{3}^{2} \)	0.002	14.56	β₃₂	−0.011
9	\( {\text{X}}_{4}^{2} \)	0.001	15.01	β₄₂	−0.065
10	X₁X₂	0.000	24.52	β₁₂	0.249
11	X₁X₃	0.015	7.63	β₁₃	0.058
12	X₁X₄	0.003	13.12	β₁₄	−0.182
13	X₂X₄	0.036	5.34	β₂₄	−0.038
R²					0.95
R² (adj)					0.91
(p value)					0.000^a
(F value)					51.26

Concentration of hexanol (×10⁻⁴ mM) X₁, enzyme amount (%) X₂, reaction time (h) X₃ and reaction temperature X₄
^aSignificant (p < 0.05)

ISSN: 2661-801X