Table 5 VNT stability data in RLMs matrix under different conditions
Nominal concentration (ng/mL) | Mean (ng/mL) | Recovery % | Precision (% RSD) |
|---|---|---|---|
Room temp. for 8 h | |||
20 | 19.35 ± 0.28 | 96.75 | 1.45 |
150 | 148.71 ± 1.62 | 99.14 | 1.09 |
400 | 396.96 ± 3.15 | 99.24 | 0.79 |
Three freeze–thaw cycles | |||
20 | 19.46 ± 0.36 | 97.31 | 1.86 |
150 | 150.47 ± 1.16 | 100.32 | 0.77 |
400 | 399.58 ± 6.02 | 99.89 | 1.51 |
Stored at 4 °C for 24 h | |||
20 | 19.43 ± 0.33 | 97.14 | 1.70 |
150 | 150.26 ± 0.98 | 100.17 | 0.65 |
400 | 398.08 ± 4.73 | 99.52 | 1.19 |
Stored at −20 °C for 30 days | |||
20 | 19.16 ± 0.59 | 95.78 | 3.08 |
150 | 149.80 ± 1.84 | 99.87 | 1.23 |
400 | 398.55 ± 5.36 | 99.64 | 1.34 |