Simultaneous determination of newly developed antiviral agents in pharmaceutical formulations by HPLC-DAD

BMC Chemistry

Table 3 Intra-day and inter-day precision and accuracy for the determination of paritaprevir (PAR), dasabuvir (DAS), ritonavir (RIT), and ombitasvir (OMB) using the proposed HPLC method

Compound	Intraday precision and accuracy (n = 3)				Interday precision and accuracy (n = 9)
Compound	Standard concentration (μg/ml)	Mean % recovery ± SD	RSD (%)^a	Er (%)^b	Mean % recovery ± SD	RSD (%)^a	Er (%)^b
PAR	5	100.53 ± 0.599	0.596	−0.526	99.17 ± 1.227	1.237	0.831
	25	99.11 ± 0.133	0.134	0.893	99.22 ± 0.396	0.399	0.781
	50	101.39 ± 0.250	0.247	−1.387	101.29 ± 0.232	0.229	−1.288
DAS	3	99.72 ± 0.324	0.326	0.727	98.78 ± 0.567	0.574	1.223
	15	100.75 ± 0.143	0.142	−0.752	100.51 ± 0.271	0.270	−0.508
	25	100.38 ± 0.17	0.17	−0.384	100.45 ± 0.352	0.350	−0.451
RIT	3.3	101.19 ± 1.183	1.169	−1.191	101.32 ± 0.996	0.983	−1.316
	16.7	100.67 ± 0.069	0.068	−0.967	101.54 ± 0.466	0.459	−1.542
	33.3	100.92 ± 0.053	0.053	−0.925	101.32 ± 1.164	1.149	−1.315
OMB	0.83	99.23 ± 0.002	0.002	0.768	99.71 ± 1.612	1.617	0.291
	4.17	100.85 ± 0.001	0.001	−0.852	100.99 ± 0.312	0.309	−0.995
	8.33	101.64 ± 0.006	0.006	−1.637	101.66 ± 0.386	0.379	−1.659

ISSN: 2661-801X