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Table 2 Summary of assay validation results

From: HPLC estimation of iothalamate to measure glomerular filtration rate in humans

Analyte (µg/mL) QC Linear range LOD R2 Intra-day (N = 6) Inter-day (N = 12) Recovery
Precision, % CV Accuracy % Precision, % CV Accuracy %
Iothalamic acid in plasma 1 1–150 0.5 0.9985 4.56 86.33 13.16 90.08  
2 9.46 112.5 12.87 102.58  
20 14.96 100.74 11.52 96.6 96.05
120 2.66 99.88 2.65 98.99 118.38
Iothalamic acid in urine 25 25–1500 5 0.9992 4.31 97.54 4.45 95.91  
40 3.96 88.49 3.86 90.91  
200 2.23 114.96 6.68 108.33 93.14
1200 1.81 109.8 8.56 101.66 111.74
  1. These values were well within the assay criteria for accuracy of nominal concentration ±15%