Determination of adapalene in gel formulation by conventional and derivative synchronous fluorimetric approaches. Application to stability studies and in vitro diffusion test

Table 2 Analytical performance data for the proposed approaches

Parameters	The first approach at 389 nm	The second approach
Parameters	The first approach at 389 nm	SDSF at 346 nm	FDSF at 312.45 nm
Linearity range (ng/mL)	2.0–14.0	2.0–14.0	2.0–14.0
Intercept (a)	−4.714	−1.212	5.171
Slope (b)	35.357	3.975	3.729
Correlation coefficient (r)	0.9999	0.9998	0.9998
SD of residuals (S_y/x)	1.59	0.34	0.31
SD of intercept (S_a)	1.34	0.29	0.26
SD of slope (S_b)	0.15	0.03	0.03
Percentage relative standard deviation, % RSD	0.42	0.88	0.76
Percentage relative error, % error	0.16	0.33	0.29
Limit of detection, LOD (ng/mL)	0.13	0.24	0.23
Limit of quantitation, LOQ (ng/mL)	0.38	0.73	0.69

ISSN: 2661-801X