Analysis of steroid hormones and their conjugated forms in water and urine by on-line solid-phase extraction coupled to liquid chromatography tandem mass spectrometry

Table 1 Tandem mass spectrometry (MS/MS) optimized parameters for the analysis of selected estrogens hormones in negative (NI) ionization mode

Hormone	Ion	SRM#1	Collision energy (V)	SRM#2	Collision energy (V)	Tube lens (V)
E3-3S	367	287	38	80	33	−98
E2-17G	447	271	31	325	28	−94
E2-3S	351	271	37	145	48	−93
E1-3S	349	269	36	145	53	−90
E2-17S	351	97	41	80	42	−96
E2-d4-3S	355	275	40	–	–	−91
E1	269	145	41	159	41	−94
E2	271	145	47	183	44	−95
EE2	295	145	48	159	38	−100
E3	287	14	44	171	37	−98
13C6-E2	277	145	48	–	–	−101

ISSN: 2661-801X