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Table 1 Tandem mass spectrometry (MS/MS) optimized parameters for the analysis of selected estrogens hormones in negative (NI) ionization mode

From: Analysis of steroid hormones and their conjugated forms in water and urine by on-line solid-phase extraction coupled to liquid chromatography tandem mass spectrometry

Hormone Ion SRM#1 Collision energy (V) SRM#2 Collision energy (V) Tube lens (V)
E3-3S 367 287 38 80 33 −98
E2-17G 447 271 31 325 28 −94
E2-3S 351 271 37 145 48 −93
E1-3S 349 269 36 145 53 −90
E2-17S 351 97 41 80 42 −96
E2-d4-3S 355 275 40 −91
E1 269 145 41 159 41 −94
E2 271 145 47 183 44 −95
EE2 295 145 48 159 38 −100
E3 287 14 44 171 37 −98
13C6-E2 277 145 48 −101