Fig. 6

Split SWV response to MAMP in a saliva buffer or b saliva. The MAMP concentrations were 0 (solid line) or 5 μg/mL (dashed line). The overlayer was treated with 1.0 mg/mL of OX1006 in 0.4 M sodium carbonate buffer (pH 10.8), containing 1.0 M NaCl and 0.1 % TX-100. The SWV procedure consisted of a 10 s wait time after application of 7 μL of sample, then (1) galvanostatic oxidation at 800 nA for 30 s; (2) SWV-1 with start voltage +0.6 V, stop voltage +0.1 V, 20 Hz frequency, 10 mV step potential and 50 mV amplitude; (3) SWV-2 with start voltage +0.1 V, stop voltage −0.4 V, 4.25 Hz frequency, 10 mV step potential and 100 mV amplitude