Figure 1

Schematic representation of the Vitrocell® 24/48 exposure system. A climatic chamber houses an exposure module that consists of a dilution/distribution system located on top of a cultivation base module. In the base module, up to 48 wells can be simultaneously exposed. The base module has a format of 8 rows × 6 columns (i.e. for 7 dilution airflows and 1 fresh air control row). The delivery of whole smoke is achieved by individual trumpets that deliver the smoke from the dilution/distribution system to the wells of the cultivation base module at a flow rate of 2 mL/min. QCMs are connected to each row of the dilution/distribution system and monitor particle deposition.