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Table 2 Calibration of the ELSD response of the four analytes based on the relationship of logarithmic values of both peak areas and analyte concentrations

From: Environmentally evaluated HPLC-ELSD method to monitor enzymatic synthesis of a non-ionic surfactant

Peak no. Compound Rt Equation R2 LOD (μg) LOQ (μg) Linear range Precision (R.S.D.%, n = 5)
         Intra-day Inter-day
1 MEG 2.18 ± 0.14 y = 1.4507x + 2.3077 0.9959 0.12 0.49 0.49-6.20 4.01 3.25
2 Amide 4.31 ± 0.30 y = 1.3716x + 2.3961 0.9986 0.10 0.59 0.59-5.90 2.54 2.24
3 Lauric 9.15 ± 0.48 y = 2.1981x - 1.5648 0.9846 2.02 5.25 5.25-26.23 3.62 2.98
4 Amide ester 17.04 ± 0.35 y = 1.5903x + 1.4929 0.9987 0.04 0.11 0.11-4.52 2.07 1.95