Table 2 Calibration of the ELSD response of the four analytes based on the relationship of logarithmic values of both peak areas and analyte concentrations
From: Environmentally evaluated HPLC-ELSD method to monitor enzymatic synthesis of a non-ionic surfactant
Peak no. | Compound | Rt | Equation | R2 | LOD (μg) | LOQ (μg) | Linear range | Precision (R.S.D.%, n = 5) | |
|---|---|---|---|---|---|---|---|---|---|
Intra-day | Inter-day | ||||||||
1 | MEG | 2.18 ± 0.14 | y = 1.4507x + 2.3077 | 0.9959 | 0.12 | 0.49 | 0.49-6.20 | 4.01 | 3.25 |
2 | Amide | 4.31 ± 0.30 | y = 1.3716x + 2.3961 | 0.9986 | 0.10 | 0.59 | 0.59-5.90 | 2.54 | 2.24 |
3 | Lauric | 9.15 ± 0.48 | y = 2.1981x - 1.5648 | 0.9846 | 2.02 | 5.25 | 5.25-26.23 | 3.62 | 2.98 |
4 | Amide ester | 17.04 ± 0.35 | y = 1.5903x + 1.4929 | 0.9987 | 0.04 | 0.11 | 0.11-4.52 | 2.07 | 1.95 |