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Table 1 Chromatographic conditions adopted for gradient HPLC separation of reaction components in the enzymatic synthesis of N-lauroyl N-methyl glucamide

From: Environmentally evaluated HPLC-ELSD method to monitor enzymatic synthesis of a non-ionic surfactant

Mobile phase Solvent A = Water: trifluoroacetic acid (0.05% w/w)
  Solvent B = Methanol  
Gradient Time (min) % Solvent A % Solvent B
  0 25 75
  5 25 75
  10 5 95
  10 5 95
  5 25 75
  10 25 75
Flow rate 1 ml/min   
Column LiChrospher® 100 RP-18 (5 μm) (LiChroCART® 125-4 HPLC cartridge) Merck, Darmstadt, Germany
Column temperature 40°C   
Detection ELSD (Alltech 3300, Alltech Associates, USA)
Injection volume 5 ul   
ELSD settings    
Temperature 38°C   
Gas flow (air) 1.3 L/min   
Gain 1   
Run time 40 min