Table 1 Precision and accuracy for the yakuchinone A and IS in rat plasma (n = 15, 5 replicates per day for 3 days)
Nominal conc. (ng/ml) | Peak area (× 104) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Intra-day | Inter-day | |||||||||||
Day 1 | Day 2 | Day 3 | ||||||||||
Mean ± SD | RSD | Accuracy | Mean ± SD | RSD | Accuracy | Mean ± SD | RSD | Accuracy | Mean ± SD | RSD | Accuracy | |
Yakuchinone A | ||||||||||||
4 | 5.72 ± 0.12 | 2.11% | 101% | 5.43 ± 0.16 | 2.98% | 94.1% | 5.31 ± 0.31 | 5.90% | 91.5% | 5.49 ± 0.27 | 4.89% | 95.4% |
80 | 94.6 ± 1.6 | 1.71% | 102% | 90.3 ± 2.5 | 2.81% | 97.7% | 93.7 ± 1.7 | 1.78% | 101% | 92.9 ± 2.7 | 2.87% | 101% |
800 | 818 ± 6 | 0.77% | 89.6% | 784 ± 4 | 0.46% | 85.8% | 813 ± 12 | 1.41% | 89.0% | 805 ± 17 | 2.13% | 88.1% |
IS | ||||||||||||
80 | 49.3 ± 1.7 | 3.52% | 102% | 48.7 ± 1.6 | 3.30% | 101% | 46.7 ± 1.5 | 3.23% | 96.3% | 48.2 ± 1.9 | 3.93% | 99.6% |