|
Parameter
|
NF
|
|---|
|
Excitation wavelength
|
340 nm
|
|
Emission wavelength
|
390 nm
|
|
Range of linearity
|
20 - 400 ng ml-1
|
|
Regression equation
|
y = 1448.447x + 101.590
|
|
Correlation coefficient (r)
|
0.9997
|
|
S
b
|
14.185
|
|
S
a
|
3.477
|
|
Confidence limit of the slope
|
1448.447 ± 45.137
|
|
Confidence limit of the intercept
|
101.590 ± 11.064
|
|
Standard error of the estimation
|
4.310
|
|
LODa
|
0.01 ng ml-1
|
|
LOQa
|
0.03 ng ml-1
|
|
Intradayb
| |
|
% RSD
|
0.692 – 0.598
|
|
Interdayc
| |
|
% RSD
|
0.688 – 0.395
|
- a Limits of detection and quantification are determined via calculations [29]:
- LOD= 3.3×SD/slope LOQ= 10×SD/slope, where SD is standard deviation of response.
- b The intraday (n = 3), average of two concentrations of NF (100, 350 ng ml−1), repeated three times within the day.
- c The interday (n = 3), average of two concentrations of NF (100, 350 ng ml−1), repeated three times in three successive days.
