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Table 4 Assay parameters and method validation obtained by applying spectrofluorimetric method for the determination of NF

From: A validated spectrofluorimetric method for the determination of nifuroxazide through coumarin formation using experimental design

Parameter NF
Excitation wavelength 340 nm
Emission wavelength 390 nm
Range of linearity 20 - 400 ng ml-1
Regression equation y = 1448.447x + 101.590
Correlation coefficient (r) 0.9997
S b 14.185
S a 3.477
Confidence limit of the slope 1448.447 ± 45.137
Confidence limit of the intercept 101.590 ± 11.064
Standard error of the estimation 4.310
LODa 0.01 ng ml-1
LOQa 0.03 ng ml-1
Intradayb  
% RSD 0.692 – 0.598
Interdayc  
% RSD 0.688 – 0.395
  1. a Limits of detection and quantification are determined via calculations [29]:
  2. LOD= 3.3×SD/slope LOQ= 10×SD/slope, where SD is standard deviation of response.
  3. b The intraday (n = 3), average of two concentrations of NF (100, 350 ng ml−1), repeated three times within the day.
  4. c The interday (n = 3), average of two concentrations of NF (100, 350 ng ml−1), repeated three times in three successive days.