Comparison of activity indexes for recognizing enzyme mutants of higher activity with uricase as model

BMC Chemistry

Table 1 Uricase activities at 0.075 mM uric acid and protein levels in lysates ( n = 30)

Candidates/parameters	A	B	C	D
Total proteins by the Bradford assay	0.63	0.66	0.65	0.66
Total proteins by the Bradford assay	(0.09)	(0.10)	(0.15)	(0.09)
Activity concentration	0.30	0.28	0.18	0.033
Activity concentration	(0.07)	(0.06)	(0.05)	(0.004)
P for normality of AC by shapiro-Wilk test	0.104	0.182	0.439	0.282
Correlation coefficient (R)	>0.63	>0.81	>0.84	>0.44
Specific activity	0.49	0.43	0.28	0.050
Specific activity	(0.09)	(0.05)	(0.05)	(0.006)
P for normality of SA by Shapiro-Wilk test	<0.001	0.310	0.972	0.031

Numbers in parentheses were SD. Correlation coefficient was that for the association of activity concentrations with levels of total proteins. R > 0.361 and > 0.464 indicated P < 0.05 and P < 0.01 for the association of activity concentrations with proteins levels, respectively. SA: specific activity; AC: activity concentration.

ISSN: 2661-801X