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Table 1 Purification of trypsin of crevalle jack pyloric caeca

From: Metal-sensitive and thermostable trypsin from the crevalle jack (Caranx hippos) pyloric caeca: purification and characterization

Step Total protein (mg) Total activity (U) Specific activity (U/mg) Yield (%) Purification (fold)
Crude extract 2 040. 0 1.863 0.9 100.0 1.0
Heat treatment 1 560. 0 1.743 1.1 85.4 1.2
Ammonium sulfate precipitation (F 0–80%) 85.6 387 4.5 20.8 5.0
Sephadex G-75 3.7 341 92.2 18.3 102.4
  1. Protein and enzyme activity were established according to Warburg and Christian [18] and Freitas-Júnior et al. [2], respectively.