Table 1 Purification of trypsin of crevalle jack pyloric caeca
Step | Total protein (mg) | Total activity (U) | Specific activity (U/mg) | Yield (%) | Purification (fold) |
|---|---|---|---|---|---|
Crude extract | 2 040. 0 | 1.863 | 0.9 | 100.0 | 1.0 |
Heat treatment | 1 560. 0 | 1.743 | 1.1 | 85.4 | 1.2 |
Ammonium sulfate precipitation (F 0–80%) | 85.6 | 387 | 4.5 | 20.8 | 5.0 |
Sephadex G-75 | 3.7 | 341 | 92.2 | 18.3 | 102.4 |