Table 2 Calibration curves, LOD, LOQ, Precision and Repeatability for ten compounds in Shen-Fu decoction
Compound name | Calibration curve | r | Linear range(ng·mL-1) | LOD(ng·mL-1) | LOQ(ng·mL-1) | Intra-day(n=6) | Inter-day(n=6) | Repeatability(n=5) |
|---|---|---|---|---|---|---|---|---|
Ginsenoside-Rb1 | Y=11.04X+181.44 | 0.9930 | 3.90~125.00 | 0.97 | 3.00 | 3.44 | 4.11 | 6.51 |
Ginsenoside-Rb2 | Y=31.68X+246.74 | 0.9882 | 3.90~125.00 | 0.97 | 3.00 | 2.23 | 3.55 | 7.01 |
Ginsenoside-Rc | Y=20.13X+25.79 | 0.9921 | 3.90~125.00 | 1.25 | 3.00 | 2.02 | 3.92 | 4.21 |
Ginsenoside-Rd | Y=13.60X+69.00 | 0.9993 | 3.90~125.00 | 0.75 | 1.95 | 4.18 | 4.71 | 3.16 |
Ginsenoside-Re | Y=18.52X+136.68 | 0.9973 | 3.90~125.00 | 0.48 | 1.95 | 3.34 | 5.06 | 4.67 |
Ginsenoside-Rf | Y=37.66X+473.14 | 0.9952 | 3.90~125.00 | 0.97 | 3.00 | 2.05 | 3.12 | 5.03 |
Ginsenoside-Rg1 | Y=52.38X+109.20 | 0.9994 | 3.90~125.00 | 0.48 | 1.50 | 2.29 | 2.49 | 4.12 |
Aconitine | Y=6193.52X-80.34 | 0.9996 | 0.03~1.25 | 0.01 | 0.04 | 2.76 | 3.51 | 4.45 |
Mesaconitine | Y=3617.22X-63.02 | 0.9939 | 0.03~1.25 | 0.01 | 0.04 | 3.97 | 3.28 | 4.96 |
Hypaconitine | Y=1207.82X+180.44 | 0.9960 | 0.19~6.24 | 0.01 | 0.04 | 2.55 | 2.42 | 4.81 |