Measurement of percentage relative survival presented as a function of deposited mass which was captured in situ of exposure [a] and as a Log
conversion [b] following a 184 minute (23 cigarettes) 3R4F smoke exposure period. [a] Average deposited mass for airflows 1.0, 4.0, 8.0 and 12.0 L/min were 22.8, 3.5, 0.8 and 0.1 μg/cm2 respectively. The calculated deposited mass IC50 was1.7 μg/cm2. [b] Using a Log10 conversion and regression analysis, the relative survival data showed a positive correlation with increased deposited mass obtained in situ (R2 = 0.84) with confidence intervals (red dash) and probability intervals (grey dash) of 95%. Results are based on three independent experiments.