Stability indicating HPLC method for the simultaneous determination of moxifloxacin and prednisolone in pharmaceutical formulations

Table 6 Stress Testing Results of Moxifloxacin and Prednisolone

Nature of stress	Storage conditions	Time (h)	Amount of moxifloxacin	Amount of prednisolone	Extent of decomposition
Nature of stress	Storage conditions	Time (h)	Remaining ± RSD (%)	Remaining ± RSD (%)	Extent of decomposition
5 M HCl	(40°C/75% RH)	16	86.3 ± 3.4 (PPI = 1.0000)	4.8 ± 3.0 (PPI = 1.0000)	Substantial
5 M NaOH	(22°C/58% RH)	0.67	100.0 ± 2.1 (PPI = 1.0000)	3.4 ± 2.7 (PPI = 1.0000)	Substantial
6% H₂O₂	(40°C/75% RH)	16	96.4 ± 1.5 (PPI = 1.0000)	88.3 ± 1.4 (PPI = 1.0000)	Substantial
Thermal	(40°C/75% RH)	144	101.3 ± 1.4 (PPI = 1.0000)	100.5 ± 1.9 (PPI = 1.0000)	None
Thermal	(40°C/75% RH)	288	97.5 ± 1.1 (PPI = 1.0000)	97.2 ± 1.5 (PPI = 1.0000)	None
Dry Heat	(105°C)	7	93.7 ± 1.7 (PPI = 1.0000)	84.1 ± 1.4 (PPI = 1.0000)	Substantial
Photolytic	Sunlight	1	88.5 ± 1.9 (PPI = 1.0000)	94.5 ± 2.5 (PPI = 1.0000)	Substantial

n = Average of 3 determinations, PPI = Peak Purity Index, Chromatographic conditions: mobile phase Methanol: 18 mM phosphate buffer 62:38, v/v, pH 2.8, Column BDS Hypersil C8 (250 X 4.6, 5 μm), flow rate 1.5 mL min^-1, injection volume 20 μL, wavelength 254 nm.

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