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Table 6 Stress Testing Results of Moxifloxacin and Prednisolone

From: Stability indicating HPLC method for the simultaneous determination of moxifloxacin and prednisolone in pharmaceutical formulations

Nature of stress Storage conditions Time (h) Amount of moxifloxacin Amount of prednisolone Extent of decomposition
Remaining ± RSD (%) Remaining ± RSD (%)
5 M HCl (40°C/75% RH) 16 86.3 ± 3.4 (PPI = 1.0000) 4.8 ± 3.0 (PPI = 1.0000) Substantial
5 M NaOH (22°C/58% RH) 0.67 100.0 ± 2.1 (PPI = 1.0000) 3.4 ± 2.7 (PPI = 1.0000) Substantial
6% H2O2 (40°C/75% RH) 16 96.4 ± 1.5 (PPI = 1.0000) 88.3 ± 1.4 (PPI = 1.0000) Substantial
Thermal (40°C/75% RH) 144 101.3 ± 1.4 (PPI = 1.0000) 100.5 ± 1.9 (PPI = 1.0000) None
(40°C/75% RH) 288 97.5 ± 1.1 (PPI = 1.0000) 97.2 ± 1.5 (PPI = 1.0000) None
Dry Heat (105°C) 7 93.7 ± 1.7 (PPI = 1.0000) 84.1 ± 1.4 (PPI = 1.0000) Substantial
Photolytic Sunlight 1 88.5 ± 1.9 (PPI = 1.0000) 94.5 ± 2.5 (PPI = 1.0000) Substantial
  1. n = Average of 3 determinations, PPI = Peak Purity Index, Chromatographic conditions: mobile phase Methanol: 18 mM phosphate buffer 62:38, v/v, pH 2.8, Column BDS Hypersil C8 (250 X 4.6, 5 μm), flow rate 1.5 mL min-1, injection volume 20 μL, wavelength 254 nm.