Stability indicating HPLC method for the simultaneous determination of moxifloxacin and prednisolone in pharmaceutical formulations

Table 2 Accuracy of the proposed HPLC method

Drugs	Spiked concentration(μg mL^-1)	Standard addition	Synthetic mixtures
Drugs	Spiked concentration(μg mL^-1)	Measured concentration(μg mL^-1) ± SD; RSD (%)	Measured concentration(μg mL^-1) ± SD; RSD (%)
Moxifloxacin	25.0	25.2 ± 0.1; 0.1	24.8 ± 0.2; 0.3
	50.0	50.4 ± 0.9; 1.4	50.6 ± 0.4; 0.6
	75.0	76.8 ± 1.1; 1.7	74.8 ± 1.1; 1.2
Prednisolone	50.0	50.9 ± 0.7; 0.7	50.1 ± 0.9; 0.1
Prednisolone	100.0	98.4 ± 0.9; 0.7	99.7 ± 0.8; 1.3
	150.0	147.9 ± 0.7; 0.2	150.0 ± 0.5; 0.7

n = Average of 5 analysis, Chromatographic conditions: mobile phase methanol: 18 mM phosphate buffer 62:38, v/v, pH 2.8, Column BDS Hypersil C8 (250 X 4.6, 5 μm), flow rate 1.5 mL min^-1, injection volume 20 μL, wavelength 254 nm.

ISSN: 2661-801X