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Table 2 Accuracy of the proposed HPLC method

From: Stability indicating HPLC method for the simultaneous determination of moxifloxacin and prednisolone in pharmaceutical formulations

Drugs Spiked concentration(μg mL-1) Standard addition Synthetic mixtures
Measured concentration(μg mL-1) ± SD; RSD (%) Measured concentration(μg mL-1) ± SD; RSD (%)
Moxifloxacin 25.0 25.2 ± 0.1; 0.1 24.8 ± 0.2; 0.3
50.0 50.4 ± 0.9; 1.4 50.6 ± 0.4; 0.6
75.0 76.8 ± 1.1; 1.7 74.8 ± 1.1; 1.2
Prednisolone 50.0 50.9 ± 0.7; 0.7 50.1 ± 0.9; 0.1
100.0 98.4 ± 0.9; 0.7 99.7 ± 0.8; 1.3
  150.0 147.9 ± 0.7; 0.2 150.0 ± 0.5; 0.7
  1. n = Average of 5 analysis, Chromatographic conditions: mobile phase methanol: 18 mM phosphate buffer 62:38, v/v, pH 2.8, Column BDS Hypersil C8 (250 X 4.6, 5 μm), flow rate 1.5 mL min-1, injection volume 20 μL, wavelength 254 nm.