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Table 1 Fatty acids composition of the forage from the 10 trials with different fertilization, using BF3/MeOH and HCl/MeOH methods (average contents of 3 different samples for each trial; significance: ns = p > 0.05, *** = p < 0.001)

From: Comparative researches on two direct transmethylation without prior extraction methods for fatty acids analysis in vegetal matrix with low fat content

  Fatty acids, mg kg -1 Significance
FA/Fertilized trials Lauric
(12:0)
Myristic
(14:0)
Palmitic
(16:0)
Stearic
(18:0)
Oleic
(18:1n9)
Linoleic
(18:2n6)
Linolenic
(18:3n3)
Arahidic
(20:0)
Behenic
(22:0)
FAs Sum A
(methods)
B
(FAs)
BF 3 /MeOH method
D1 33.4 73.7 1644.4 237.1 465.8 1167.9 665.7 132.2 129.2 4549.4 ns ***
D2 19.1 55.2 1876.3 239.9 564.7 1712.3 1605.9 127.0 103.9 6304.3 ns ***
D3 23.3 79.0 2257.6 424.1 736.8 2681.0 1546.9 169.4 154.6 8072.7 ns ***
D4 18.6 65.0 2413.7 509.8 851.8 2909.5 1988.1 207.5 150.5 9114.4 ns ***
D5 15.6 47.8 1729.9 295.8 565.0 1929.3 757.0 202.1 122.2 5664.7 ns ***
D6 15.3 51.4 1764.1 282.9 513.5 1471.4 749.4 125.1 76.3 5049.5 ns ***
D7 24.0 59.2 1474.4 200.3 423.2 1027.3 615.4 124.4 105.8 4053.9 ns ***
D8 18.4 55.9 1535.4 189.4 441.7 1069.6 608.2 106.8 96.0 4121.4 ns ***
D9 20.6 52.8 1531.5 179.0 352.2 721.6 509.7 93.2 77.5 3538.1 ns ***
D10 18.4 46.2 1378.7 156.4 298.4 673.4 475.0 104.2 94.2 3245.0 ns ***
Average SDV 1.9 3.2 61.4 14.7 15.0 10.7 7.9 6.6 13.3 131.4   
Average RSDV % 25.5 9.9 4.3 7.8 6.2 1.9 1.4 7.4 12.2 4.0   
HCl/MeOH method
D1 14.2 43.1 1642.0 236.7 577.5 1349.6 523.8 142.0 70.5 4599.4 ns ***
D2 8.8 17.6 1805.7 232.3 620.9 1673.7 1310.5 115.1 77.1 5861.6 ns ***
D3 2.8 13.5 2208.5 518.3 974.6 2781.7 1223.9 131.0 156.3 8010.7 ns ***
D4 4.8 44.2 2622.6 542.2 970.9 3252.0 1808.1 230.5 238.9 9714.2 ns ***
D5 9.6 12.4 1876.8 341.9 782.5 2175.4 728.3 252.8 160.1 6339.9 ns ***
D6 4.1 26.9 1731.4 271.3 534.3 1388.7 612.0 136.7 118.1 4823.6 ns ***
D7 5.4 24.1 1449.8 178.2 438.1 1146.9 489.1 108.5 105.9 3946.0 ns ***
D8 12.3 42.5 1674.6 223.7 499.4 1241.3 536.2 124.6 135.9 4490.6 ns ***
D9 15.1 38.8 1401.4 188.6 328.8 721.2 423.6 67.9 108.9 3294.3 ns ***
D10 8.2 49.3 1467.6 186.9 444.7 805.2 402.6 100.7 119.1 3584.4 ns ***
Average SDV 4.9 4.2 218.1 72.2 189.1 115.9 68.6 6.7 13.5 669.4   
Average RSDV% 130.6 18.0 13.2 29.5 54.3 17.9 11.6 7.7 13.9 18.1