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Table 4 Summary of stress degradation studies of prednisolone acetate and chloramphenicol in combination.

From: Stress degradation studies and development of stability-indicating TLC-densitometry method for determination of prednisolone acetate and chloramphenicol in their individual and combined pharmaceutical formulations

Degradation conditions % Degradation of 1 % Degradation of 2 Rf of degraded products
Acidic hydrolysisa    
1N HCl 100 31.8 0.01, 0.04, 0.17, 0.29,0.78,
0.81
5N HCl 100 82.7 0.01, 0.02, 0.04, 0.17, 0.29,
0.38,0.58, 0.78
Basic hydrolysisa    
0.1 N NaOH 100 100 0.01, 0.02, 0.17,0.29, 0.50, 0.79,0.85
1N NaOH 100 100 0.01, 0.02, 0.03, 0.29,0.44
5N NaOH 100 100 0.01, 0.02, 0.31
Neutral hydrolysisa    
H2O 74.52 17.9 0.01, 0.17, 0.29, 0.79
Wet heatinga 17 36.9 0.01, 0.17, 0.29, 0.50,0.79
Dry heating 0 0 0.01, 0.26, 0.47,0.73,0.79
Photochemical 85.808 17.1 0.01, 0.47, 0.49,0.50,0.58, 0.69,0.73
Oxidation    
35%v/v H2O2 a 30.56 7.20 0.01, 0.12, 0.31, 0.55
At room temp 9.029 13.4 0.01, 0.12, 0.23,0.31, 0.55
  1. aReflux in parallel synthesizer for two hours at 80°C