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Table 2 Product distribution of the RNA cleavage reaction in trans-(±)-cyclohexane-1,2-diaminea

From: Cleavage of pyrene-stabilized RNA bulge loops by trans-(±)-cyclohexane-1,2-diamine

  RNA 1 (%) Duplex 1/2 (%) Duplex 1/7 (%) Duplex 1/8 (%)
Full-length 70.3 ± 5.1 88.6 ± 2.3 75.6 ± 6.5 68.9 ± 1.7
n-1 4.0 ± 0.5 < 1 6.9 ± 1.0 4.4 ± 2.1
n-2 3.8 ± 0.4 < 1 < 1 < 1
n-3 4.5 ± 0.5 < 1 < 1 < 1
n-8 < 1 < 1 < 1 3.6 ± 0.8
n-9 < 1 < 1 < 1 4.7 ± 0.2
n-10 < 1 < 1 3.4 ± 1.2 4.0 ± 0.1
n-11 3.6 ± 0.3 < 1 < 1 < 1
n-12 3.1 ± 1.0 < 1 < 1 < 1
n-13 2.9 ± 1.1 4.2 ± 0.9 2.6 ± 0.3 4.6 ± 2.3
n-14 3.6 ± 1.3 3.6 ± 0.9 3.3 ± 1.1 4.8 ± 1.1
  1. a. Buffer: 0.5 M trans-(±)-cyclohexane-1,2-diamine, 20 mM NaCl, and 1 mM Tris-EDTA (37°C, pH 8.0, 18 h).