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Table 2 Product distribution of the RNA cleavage reaction in trans-(±)-cyclohexane-1,2-diaminea

From: Cleavage of pyrene-stabilized RNA bulge loops by trans-(±)-cyclohexane-1,2-diamine

 

RNA 1 (%)

Duplex 1/2 (%)

Duplex 1/7 (%)

Duplex 1/8 (%)

Full-length

70.3 ± 5.1

88.6 ± 2.3

75.6 ± 6.5

68.9 ± 1.7

n-1

4.0 ± 0.5

< 1

6.9 ± 1.0

4.4 ± 2.1

n-2

3.8 ± 0.4

< 1

< 1

< 1

n-3

4.5 ± 0.5

< 1

< 1

< 1

n-8

< 1

< 1

< 1

3.6 ± 0.8

n-9

< 1

< 1

< 1

4.7 ± 0.2

n-10

< 1

< 1

3.4 ± 1.2

4.0 ± 0.1

n-11

3.6 ± 0.3

< 1

< 1

< 1

n-12

3.1 ± 1.0

< 1

< 1

< 1

n-13

2.9 ± 1.1

4.2 ± 0.9

2.6 ± 0.3

4.6 ± 2.3

n-14

3.6 ± 1.3

3.6 ± 0.9

3.3 ± 1.1

4.8 ± 1.1

  1. a. Buffer: 0.5 M trans-(±)-cyclohexane-1,2-diamine, 20 mM NaCl, and 1 mM Tris-EDTA (37°C, pH 8.0, 18 h).