Table 2 Assay parameters for the determination of ALD by the three proposed methods
parameter | Method I | Method II | Method III |
|---|---|---|---|
Standard conc. (μg/mL) | 200 | 400 | 300 |
Borate buffer pH | 10.7 ± 0.2 | 10.5 ± 0.5 | 10.0 ± 0.5 |
Borate buffer volume (mL) | 1 ± 0.5 | 1 ± 0.5 | 1 ± 0.5 |
Reagent conc. | 0.2% w/v | 0.3% v/v | 0.3% v/v |
Reagent volume (mL) | 1.2 ± 0.2 | 1.2 ± 0.2 | 1.0 ± 0.5 |
Temperature (°C) | 70 ± 5 | 60 ± 5 | Room temp. |
Time (min) | 25 ± 5 | 15 ± 5 | 5 |
Stability of the product (min) | 60 | At least 60 | At least 60 |
Diluting solvent | methanol | water | water |
λ max (nm) | 472 | 378 | 374 |