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Table 2 Assay parameters for the determination of ALD by the three proposed methods

From: Validated spectrophotometric methods for determination of Alendronate sodium in tablets through nucleophilic aromatic substitution reactions

parameter Method I Method II Method III
Standard conc. (μg/mL) 200 400 300
Borate buffer pH 10.7 ± 0.2 10.5 ± 0.5 10.0 ± 0.5
Borate buffer volume (mL) 1 ± 0.5 1 ± 0.5 1 ± 0.5
Reagent conc. 0.2% w/v 0.3% v/v 0.3% v/v
Reagent volume (mL) 1.2 ± 0.2 1.2 ± 0.2 1.0 ± 0.5
Temperature (°C) 70 ± 5 60 ± 5 Room temp.
Time (min) 25 ± 5 15 ± 5 5
Stability of the product (min) 60 At least 60 At least 60
Diluting solvent methanol water water
λ max (nm) 472 378 374