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Table 2 Method validation data for clematichinenoside AR and its related impurities

From: Development and validation of a chromatographic method for determining Clematichinenoside AR and related impurities

Parameters Imp-1 Imp-2 AR Imp-3 Imp-4 Imp-5
System suitability  
RTb 2.533±0.008 3.934±0.016 9.793±0.041 10.518±0.037 11.206±0.037 18.449±0.011
RRTb,c 0.259±0.0003 0.402±0.0002 1.000±0.0000 1.074±0.0008 1.144±0.0012 1.884±0.0071
RSb,c   6.79±0.050 18.33±0.175 1.99±0.008 2.06±0.008 26.91±0.381
ASb,c 1.124±0.004 1.047±0.057 0.838±0.003 0.964±0.003 0.974±0.004 1.012±0.0091
α b, c   1.872±0.434 1.872±0.434 1.080±0.008 1.070±0.006 1.686±0.056
K’ b, c 0.693±0.005 1.630±0.011 5.547±0.028 6.032±0.025 6.492±0.025 11.335±0.007
NTP b, c 4009±44 3889±107 10299±174 14992±321 19418±130 248207±2222
Linearity  
r2 0.9998 0.9994 0.9994 0.9998 0.9994 0.9992
Slope 2.9525 2.9072 2.9442 3.1068 3.5708 4.9601
Intercept −0.0324 −2.3100 0.2528 −0.9281 −2.0499 0.3703
Precision(RSD n=6,,%) 0.76 1.04 0.22 0.76 0.45 1.63
LOD(μg·ml-1) 0.55 1.87 0.49 0.57 0.53 1.04
LOQ(μg·ml-1) 1.10 2.80 0. 99 1.13 1.06 2.08
Accuracy (80%) 99.88% 99.54% 98.73% 100.29% 95.60% 104.76%
Accuracy (100%) 99.30% 100.36% 99.44% 100.23% 99.56% 96.15%
Accuracy(120%) 101.23% 96.50% 103.56% 102.67% 98.08% 98.08%
  1. RT, retention time/min; RRT, relative retention time; R, resolution; A, tailing factor; a, selectivity; K’, capacity; NTP, number of theoretical plates. bAverage of five determinations .cMean±SD (n = 5).