Table 2 Method validation data for clematichinenoside AR and its related impurities

System suitability

RT^b

2.533±0.008

3.934±0.016

9.793±0.041

10.518±0.037

11.206±0.037

18.449±0.011

RRT^b,c

0.259±0.0003

0.402±0.0002

1.000±0.0000

1.074±0.0008

1.144±0.0012

1.884±0.0071

R_S^b,c

6.79±0.050

18.33±0.175

1.99±0.008

2.06±0.008

26.91±0.381

A_S^b,c

1.124±0.004

1.047±0.057

0.838±0.003

0.964±0.003

0.974±0.004

1.012±0.0091

α ^{b, c}

1.872±0.434

1.872±0.434

1.080±0.008

1.070±0.006

1.686±0.056

K’ ^{b, c}

0.693±0.005

1.630±0.011

5.547±0.028

6.032±0.025

6.492±0.025

11.335±0.007

NTP ^{b, c}

4009±44

3889±107

10299±174

14992±321

19418±130

248207±2222

Linearity

r²

0.9998

0.9994

0.9994

0.9998

0.9994

0.9992

Slope

2.9525

2.9072

2.9442

3.1068

3.5708

4.9601

Intercept

−0.0324

−2.3100

0.2528

−0.9281

−2.0499

0.3703

Precision(RSD n=6,,%)

0.76

1.04

0.22

0.76

0.45

1.63

LOD(μg·ml-1)

0.55

1.87

0.49

0.57

0.53

1.04

LOQ(μg·ml-1)

1.10

2.80

0. 99

1.13

1.06

2.08

Accuracy (80%)

99.88%

99.54%

98.73%

100.29%

95.60%

104.76%

Accuracy (100%)

99.30%

100.36%

99.44%

100.23%

99.56%

96.15%

Accuracy(120%)

101.23%

96.50%

103.56%

102.67%

98.08%

98.08%