Enzyme preparation | % Activity recovered |
---|
Straight from the bottle | - |
EPRP | 97 |
ECCN 1 | 93.2 |
ECCN 2 | 91.6 |
ECCN 3 | 94.9 |
ECCN 4 | 92.4 |
ECCN 5 | 90.7 |
- The EPRP and ECCNs were suspended back in 50 mM phosphate buffer, gently vortexed and then separated using a magnetic separator. The superparamagnetic nanoparticles were removed and the clear supernatant containing the dissolved alpha chymotrypsin was collected. The activity of the recovered protein was determined by measuring the initial rates for the hydrolysis of N-benzoyl-L-tyrosine ethyl ester. Each measurement was done in duplicate and the difference between each reading was within 3%.