Table 1 Effect of experimental parameters on the number of theoretical plates of the studied compounds
Parameter | No. of theoretical plates (N) | Resolution (Rs) | |||
|---|---|---|---|---|---|
MEB | SUL | MEB | SUL | ||
Ratio of organic modifier A/B | 40/60 | 1199 | 2521 | 3.20 | 0.345 |
45/55 | 3407 | 4691 | 6.00 | 3.95 | |
50/50 | 2867 | 1824 | 5.75 | 1.77 | |
55/45 | 1658 | 911 | 3.97 | 1.20 | |
60/40 | 1255 | 1348 | 3.97 | 1.46 | |
70/30 | 1946 | 834 | 4.90 | 6.19 | |
75/25 | 1487 | 825 | 4.20 | 0.99 | |
pH | 3.0 | 2687 | 1778 | 5.04 | 1.55 |
3.5 | 1138 | 1510 | 3.50 | 1.52 | |
4.0 | 3462 | 4659 | 6.16 | 3.70 | |
4.5 | 1716 | 2366 | 5.10 | 3.10 | |
5.0 | 1345 | 1630 | 5.33 | 3.10 | |
Ionic strength of phosphate buffer [M] | 0.0075 | 2935 | 2315 | 5.08 | 2.24 |
0.01 | 3451 | 4632 | 6.16 | 3.72 | |
0.02 | 1614 | 1251 | 5.60 | 2.62 | |
0.03 | 1982 | 1150 | 6.20 | 2.50 | |
0.04 | 1394 | 950 | 5.30 | 2.40 | |
Flow rate mL/min | 0.50 | 1297 | 1783 | 3.6 | 1.5 |
0.75 | 2356 | 3252 | 5.20 | 2.50 | |
1.00 | 3442 | 4621 | 6.20 | 3.90 | |
1.20 | 1864 | 2534 | 4.75 | 3.10 | |
1.50 | 1534 | 1963 | 5.80 | 3.40 | |