Skip to main content

Table 1 Validation parameters for first and ratio first derivative spectroscopic methods

From: Simultaneous determination of moxifloxacin and cefixime by first and ratio first derivative ultraviolet spectrophotometry

 Parameters assessed Method D1* Method RD1**
  MOX CEF MOX CEF
Beer’s law range (μg /mL ) 1-16 1-16 1-15 1-15
Wavelength (nm) 287.0 317.9 359.3 269.6
Correlation Coefficent(r2) 0.9992 0.9992 0.9991 0.9993
Slope 0.00362 0.00114 0.1807 0.0109
Intercept 5.08x10-4 −4.32x10-5 −0.0196 −0.0016
LOD 0.22 0.32 0.24 0.14
LOQ 0.75 0.95 0.70 0.43
Intraday precision (%RSD) 0.87 1.35 0.92 0.80
Interday precision(%RSD) 1.56 1.79 1.38 1.18
  1. * First derivative Spectrophotometric method. **First derivative of the ratio spectra.
  2. MOX-Moxifloxacin HCl, CEF- Cefixime trihydrate. %RSD : Percent Relative Standard Deviation.