Development of a method for environmentally friendly chemical peptide synthesis in water using water-dispersible amino acid nanoparticles

Table 3 Grinding condition and particle size of water-dispersible Fmoc-Ala-OH nanoparticles^a

Entry	Detargent agent	Bead Size [mm]	Particle Size [nm]
1	0.4% Triton X-100 solution	0.5	292 ± 119
2	0.2% Triton X-100 solution	0.5	217 ± 89
3	0.2% Triton X-100 solution	1.0	362 ± 157
4	0.1% Triton X-100 solution	1.0	484 ± 225
5	0.01% Triton X-100 solution	1.0	2581 ± 954

^aAn aqueous dispersion of nanoparticulate Fmoc-Ala-OH was prepares by grinding using a planetary ball mill. An agate jar was charged with precleaned zirconium oxide beads, Fmoc-Ala-OH, and detergent solution. The batch was rolled for 3 hr. After grinding, the beads were removed by filtration with water.

ISSN: 2661-801X