Table 3 Typical sequence of particular steps of an SIC program controlling separation and quantification of HTZ and PRP
Action description | Time (s) | Valve position | Pump action | Flow rate (μL/s) | Volume (μL) |
|---|---|---|---|---|---|
Filling syringe with mobile phase | 7 | Check valve in the syringe pump | Aspirate | 150 | 1000 |
Column conditioning with mobile phase | 33 | Central port in the SV with port-2 | Dispense | 30 | 1000 |
Filling syringe with mobile phase for elution | 20 | Check valve in the syringe pump | Aspirate | 150 | 3000 |
Standard/sample loading | 4.0 | 3-10 | Aspirate | 10 | 40 |
Separation with UV detection | 101 | Central port in the SV with port-2 | Dispense | 30 | 3040 |
Total | 165 | - | - | - | 4040 |