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Figure 3 | Chemistry Central Journal

Figure 3

From: Sequential injection chromatography with a miniaturized multi-channel fiber optic detector for separation and quantification of propranolol and hydrochlorothiazide

Figure 3

Sequential injection chromatogram for four replicate measurements of 5, 25 and 50 μg/mL HTZ and 25, 100 and 200 μg/mL PRP for calibration purpose. Optimum conditions: C18 monolithic column (4.6 × 50 mm), a mobile phase composition of 75 mmol/L phosphate:acetonitrile (70:30, v/v) at pH 3.0, flow rate 30 μL/s, sample volume 40 μL, UV detection at 270 nm for HTZ and 290 nm for PRP.

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