Table 8 Activation parameters for the hydride transfer catalyzed by Escherichia coli dihydrofolate reductase in various solvents
From: On the curvature in logarithmic plots of rate coefficients for chemical reactions
Solvent | a |
| Δu TS / kcal mol-1 | σ |
|
|---|---|---|---|---|---|
H2O | 1 | 2.73 108 (1.26 108) | 8.29 (0.23) | 1.65 10-2 (1.14 10-2) | 7.02 10-2 (1.92 10-3) |
MeOH 17% | 24 (18.08) | 1.46 104 (4.78 104) | 20.95 (9.92) | 8.20 10-3 (1.03 10-2) | 0.67 (0.11) |
MeOH 33% | 1 | 1.46 107 (8.52 106) | 6.72 (0.27) | 1.35 10-2 (1.41 10-2) | 8.66 10-2 (3.60 10-3) |
MeOH 50% | 1 | 3.04 106 (3.89 106) | 6.37 (0.42) | 3.67 10-2 (1.74 10-2) | 8.98 10-2 (6.18 10-3) |
glycerol 17% | 1 | 3.45 109 (8.72 108) | 9.74 (0.14) | 7.22 10-2 (7.56 10-3) | 5.98 10-2 (8.51 10-4) |
glycerol 33% | 96 (18.15) | 1.06 103 (1.13 103) | 62.22 (10.18) | 2.39 10-2 (7.37 10-3) | 0.90 (3.09 10-2) |
glycerol 50% | 169 (20.18) | 3.87 102 (5.56 101) | 104.67 (11.37) | 3.37 10-2 (8.57 10-3) | 0.94 (1.09 10-2) |
sucrose 17% | 3 (1.00) | 4.65 106 (9.44 106) | 8.80 (0.48) | 1.96 10-2 (1.76 10-2) | 0.20 (5.53 10-2) |
sucrose 30% | 108 (44.96) | 4.38 102 (1.10 103) | 64.78 (24.83) | 5.79 10-2 (9.23 10-3) | 0.97 (6.75 10-2) |
