Table 8 Activation parameters for the hydride transfer catalyzed by Escherichia coli dihydrofolate reductase in various solvents
From: On the curvature in logarithmic plots of rate coefficients for chemical reactions
| Solvent | a |
| Δu TS / kcal mol-1 | σ |
|
|---|---|---|---|---|---|
| H2O | 1 |
2.73 108 (1.26 108) |
8.29 (0.23) |
1.65 10-2 (1.14 10-2) |
7.02 10-2 (1.92 10-3) |
| MeOH 17% |
24 (18.08) |
1.46 104 (4.78 104) |
20.95 (9.92) |
8.20 10-3 (1.03 10-2) |
0.67 (0.11) |
| MeOH 33% | 1 |
1.46 107 (8.52 106) |
6.72 (0.27) |
1.35 10-2 (1.41 10-2) |
8.66 10-2 (3.60 10-3) |
| MeOH 50% | 1 |
3.04 106 (3.89 106) |
6.37 (0.42) |
3.67 10-2 (1.74 10-2) |
8.98 10-2 (6.18 10-3) |
| glycerol 17% | 1 |
3.45 109 (8.72 108) |
9.74 (0.14) |
7.22 10-2 (7.56 10-3) |
5.98 10-2 (8.51 10-4) |
| glycerol 33% |
96 (18.15) |
1.06 103 (1.13 103) |
62.22 (10.18) |
2.39 10-2 (7.37 10-3) |
0.90 (3.09 10-2) |
| glycerol 50% |
169 (20.18) |
3.87 102 (5.56 101) |
104.67 (11.37) |
3.37 10-2 (8.57 10-3) |
0.94 (1.09 10-2) |
| sucrose 17% |
3 (1.00) |
4.65 106 (9.44 106) |
8.80 (0.48) |
1.96 10-2 (1.76 10-2) |
0.20 (5.53 10-2) |
| sucrose 30% |
108 (44.96) |
4.38 102 (1.10 103) |
64.78 (24.83) |
5.79 10-2 (9.23 10-3) |
0.97 (6.75 10-2) |
