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Table 4 Substrate specificity of recombinant AlEH1.

From: Cloning and functional characterization of a fructan 1-exohydrolase (1-FEH) in edible burdock (Arctium lappaL.)

Substrate

DP

Relative activity

Sucrose

2

0.2

Inulobiose

2

12

Inulotriose

3

41

1-kestose

3

100

6-kestose

3

2

Neokestose

3

7

Nystose

4

66

Fructosylnystose

5

97

Inulin

 

42

Levan

 

0

  1. Relative activity is expressed as the percentage of the activity with 1-kestose.
  2. Final concentration of each substrate in reaction mixture is 10 mM except for levan.
  3. Concentration of the levan in reaction mixture is 0.5%.
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BMC Chemistry

ISSN: 2661-801X

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