Figure 1

Precipitation of enzymatic activities with organic solvents: Three different precipitants (acetone, dimethoxyethane (DME) and n-propanol, 5 ml each) were added to crude Pectinex™ Ultra SP-L (containing pectinase, xylanase and cellulase activity) (1 ml) for complete precipitation of proteins at 4°C as described in the experimental section. The percentage activities of the enzymes in the precipitate were calculated by taking the initial activities as 100%. The experiments were carried out in triplicate and error bars represent the percentage error in each set of readings. -■-: pectinase, -□-: xylanase, --: cellulase.