Atom transfer radical polymer-modified paper for improvement in protein fixation in paper-based ELISA

A newly modified paper-based enzyme-linked immunosorbent assay (P-ELISA) was established by immobilizing more proteins on the paper surface through an atom transfer radical polymerization (ATRP) reaction. In addition, introducing graphene oxide (GO) sheets, Au nanoparticles (AuNps) and two primary antibodies (Ab1s) led to signal amplification and cost reduction. Electronic supplementary material The online version of this article (10.1186/s13065-019-0622-7) contains supplementary material, which is available to authorized users.


Introduction
An enzyme-linked immunosorbent assay (ELISA) is an effective and powerful method for protein detection and has been widely used for immunoassays, especially those for detecting and measuring trace biomarkers in complex samples. However, the poor limit of detection (LOD), the need for expensive and large amounts of Ab1s, the requirement for multiple incubation steps and the need for many washing steps have increasingly limited its application. P-ELISA was first proposed in 2010 by Whitesides' group as a promising platform and has attracted increasing attention due to its simplicity, speediness and low cost. Despite its popularity, unsatisfactory detection is its main disadvantage and has not been solved. The main reason for this issue is that proteins have a low adhesion to paper [1][2][3].
To solve this problem, several groups have performed many studies and partially solved the problem. The Chen group increased the signal by employing multi-enzyme carbon nanospheres, the Zhao group enhanced the signal by plasma treatment of paper for protein immobilization, and the Dong group increased the sensitivity by high loading of MnO 2 nanowires on graphene paper [4][5][6][7]. Although these new modifications achieved some improvements, the problem of protein immobilization on paper has not been comprehensively solved. In our study, paper was modified by the ATRP reaction, and many hair-like polymeric chains were generated on its surface to combine with a large number of objects to be detected [8,9]. Untreated paper is very thin and offers few functional groups. Thus, proteins are difficult to attach to paper surfaces, and even attached proteins are easily washed away. In this work, a new and effective protein immobilization method was studied by introducing an ATRP reaction. As a widely used method of aggregation, ATRP can generate many branches on the paper surface and sharply increase the surface area [10,11]. P-ELISA modified by an ATRP reaction (AP-ELISA) can bind proteins more firmly because of the many polymer chains, and the effect is even more pronounced for small molecules [12,13].
To further amplify the detection signal, we introduced GO sheets and AuNps. Graphene is a novel, one-atom thick, two-dimensional graphitic carbon system that has the advantages of a unique structure and easy conjugation with proteins without degrading their biological activity [14,15]. AuNps are an excellent biological carrier because of its high surface-to-volume ratio and wide range of sizes (1 to 200 nm) [16,17]. Qi et al. BMC Chemistry (2019) 13:110

Preparation of the initiator
The use of untreated paper is greatly restricted because of its low surface energy and strong hydrophobicity. Thus, hydrophilic modification by ATRP is necessary before it is used for ELISA [18,19]. Briefly, the necessary step is preparation of the initiator according to the following protocol. First, 11-mercapto-1-undecanol was dissolved in tetrahydrofuran. Second, pyridine and nitrogen gas were added. Third, 2-bromoiso-butyryl bromide was gently added after the solution cooled. After stirring for 4 h, the mixed liquor was filtered and dried under nitrogen. Finally, the initiator was obtained and stored at 4 °C under nitrogen for use.

Preparation of paper-ATRP-protein
The paper was placed in an eppendorf micro test tube (EP tube) containing 500 μL of initiator, and then was placed on a rotating instrument overnight. After washing with methanol, the ATRP reaction mixture (2 M GMA, 0.02 M CuCl, 0.03 M N, N, N′, N′,N″-pentamethyl diethylenetriamine, and 0.001 M CuCl 2 dissolved in cyclohexanol) and 0.03 M glucose were added and shaken at RT for 24 h. To investigate the difference in the binding ability of paper to proteins before and after ATRP modification, an amino acid fragment (20-100) from hepatic erythropoietin with a molecular weight of approximately 8.8 kDa was used as a model.
Under the action of the polymer chains, more proteins were firmly attached to the surface of the paper and were not washed off easily because of the blocking action [20][21][22][23][24][25][26].

Preparation of AuNps and Ab1-AuNps-Ab1′
The AuNps in our research were made using a method similar to the conventional procedure. First, all glassware used in the experiment was thoroughly washed with aqua regia (three parts HCl, one part HNO 3 ), rinsed in doubly distilled water, and oven-dried prior to use. Second, 100 mL of 0.01% (mass percentage) HAuCl 4 ·4H 2 O in doubly distilled water was brought to a boil under continuous stirring. Then, 2.5 mL of l % (mass percentage) sodium citrate solution was quickly added, stirred, and kept boiling for another 15 min. The solution colour changed from grey to blue, then purple, and finally to wine red during this period. Boiling was sustained for 10 min, the heating source was removed, the suspension was stirred for another 15 min, and it was stored in dark bottles at 4 °C for use.
To increase the combination ratio of Ab1 s and reduce the cost, AuNps and double Ab1 s were introduced in this study [27][28][29][30][31]. First, 30 μg of Ab1 and assisted primary antibody (Ab1′, specifically GST-Ab1) were added to a 1 mL suspension of AuNps. Following the literature [32], the molar ratio of Ab1:Ab1′ was 1:10. After incubation at RT for 2 h with gentle stirring, Ab1 and Ab1′ were adsorbed onto the AuNps surface through a combination of interactions. Second, after blocking with BSA, the Ab1-AuNps-Ab1′ complex was centrifuged at 13,300 rpm. Third, after the supernatant was discarded, Ab1-AuNps-Ab1 was obtained at the bottom. Finally, the conjugate was dispersed in PBS containing 1% BSA to increase its stability and minimize nonspecific adsorption during storage at 4 °C. The TEM images of AuNps, Ab1-AuNps and Ab1-AuNps-Ab1′ are displayed in Fig. 1 [33][34][35].

Preparation of Ab2-GO-HRP
The GO used in this study was prepared using a modified Hummers method [36][37][38]. The secondary antibody-GOhorseradish peroxidase (Ab2-GO-HRP) conjugate was synthesized according to the following protocol. First, 50 mg of ClCH 2 COONa and 50 mg of NaOH were added to a 1 mg mL −1 GO suspension. After bath sonication for 1.5 h, the mixed liquor was washed three times. Second, MES buffer containing 400 mM EDC and 200 mM NHS was added. After 30 min of reaction, a homogeneous black suspension was obtained. Third, after washing 3 times, the polymer was suspended in PBS and stirred for 4 h at RT. Finally, Ab2-GO-HRP was resuspended in To ensure that HRP and Ab2 were successfully bonded to GO, XPS analysis was conducted (shown in Additional file 1: Fig. S1).
The Ab2-GO-HRP spectrum exhibits a single sharp N 1 s peak centred at 399.6 eV (curve A in Additional file 1: Fig. S1), while the spectrum of GO does not (curve B in Additional file 1: Fig. S1). This result indicates that the dark spots originate from Ab2-GO-HRP [4,39,40].

Results and discussion
We successfully modified an AP-ELISA method by introducing an ATRP reaction, AuNps and GO (shown in Fig. 3). Compared with unmodified paper, the ATRPmodified paper demonstrated greater ability to prevent the loss of small molecules and increased the detection signal of the target protein or peptide.
In order to investigate the properties of treated paper, the following experiments were carried out. The results showed that compared with the unmodified paper, the paper modified by ATRP had a stronger binding to protein and exhibited a 70% increase in binding capacity (shown in Table 1) [23][24][25][26][27].
The load increase of amino acid fragment on the membrane was measured using the following Eq.
where m 1 is the amount of protein adsorbed by raw paper in P-ELISA method, and m 2 is the amount of protein adsorbed by ATRP modified paper in of AP-ELISA method.
To verify the reliability of the newly developed AP-ELISA method, patient serum samples containing AFP collected from Beijing University Cancer Hospital (Beijing, China) were examined by the AP-ELISA method and the exiting P-ELISA method. The results are shown in Fig. 4.
As shown in Fig. 4, the samples in the vertical columns contained the same amount of AFP, and the samples in   . 4 A fourfold dilution series of AFP was detected using AP-ELISA (a) and P-ELISA method (b). The paper used in control 1 is treated paper and in control 2 is pristine paper the horizontal rows represented a fourfold dilution series. AFP was detected in the first seven columns using the AP-ELISA method, while it was detected in only the first three columns using P-ELISA. Therefore, the modified AP-ELISA strategy was 256 times more sensitive than the P-ELISA method. The calibration curves of AP-ELISA and P-ELISA were shown in Additional file 1: Fig. S2. The improvement in the LOD for the AP-ELISA strategy is a consequence of the step-by-step signal amplification resulting from introducing ATRP, GO sheets and AuNps [41,42]. Because the paper has strong adhesion ability after treatment, it can attach more antigens than the untreated paper, which is the first amplification. Then, AuNps conjugate with several copies of Ab1 and Ab1′, which is the second amplification [43]. Finally, GO sheets conjugate more HRP than Ab2, resulting in an increase in the ratio of HRP to Ab2, which is the third amplification (shown in Fig. 5).
Cost reduction is another significant improvement in AP-ELISA over P-ELISA. Low cost is one of the characteristics of this method. In this study, a portion of the expensive Ab1 was replaced by the cheap Ab1′, and this significantly decreased the cost of AP-ELISA. To our knowledge, this report is the first on a modified P-ELISA method that amplifies the signal three times and reduces the cost by introducing two kinds of Ab1.
The advantages of AP-ELISA are shown in Table 2.
Escherichia coli serotype O157:H7 (E. coli O157:H7) is an epidemic human pathogen responsible for countless deaths [1,2]. Even now, this situation still exists in undeveloped areas. It is urgent to detect E. coli O157:H7 accurately and simply. A total of 0.5 g of lettuce picked from a local garden was treated by grinding, washing and filtering. The concentrations of E. coli O157:H7 in successive filtrates were 10 7 , 10 6 , 10 5 , 10 4 , and 10 3 CFU/mL. The cyan-magenta-yellow (CMY) grey value is expressed as the mean ± standard deviation ( x ± SD). The Shapiro-Wilk test and the Kolmogorov-Smirnov test were used to verify the assumption of normality. Analysis of variance (ANOVA) and t test were used, and all analyses used a two-sided 0.05 significance level. The results showed that the standard calibration curve of vegetables was established over the range of 10 3 -10 7 CFU/mL (shown in Fig. 6).
These findings show that the newly modified AP-ELISA method was effective in complex matrix detection, and the LOD reached 1 × 10 3 CFU/mL.

Conclusion
In summary, we successfully developed an ultrasensitive AP-ELISA method with triple signal amplification and cost reduction by introducing ATRP-modified paper, GO sheets, AuNps and double Ab1 s. The results suggest that the AP-ELISA method is feasible for detecting target proteins, especially small molecules. Moreover, compared to the existing P-ELISA method, the AP-ELISA method  is 256-fold more sensitive, and the cost is only one-third of the original method. To our knowledge, this report is the first using ATRP as the protein immobilization method for P-ELISA. More importantly, this immobilization strategy can be applied not only to P-ELISA but also to other biological immunoassay methods and biosensors based on the covalent immobilization of protein on paper.

Additional file
Additional file 1. Additional figures.