Design, synthesis and biological activity of hydroxybenzoic acid ester conjugates of phenazine-1-carboxylic acid

We prepared 16 novel hydroxybenzoic acid ester conjugates of phenazine-1-carboxylic acid (PCA) and investigated their biological activity. Most of the synthesized conjugates displayed some level of fungicidal activities in vitro against five phytopathogenic fungi. Nine conjugates 5b, 5c, 5d, 5e, 5h, 5i, 5m, 5n and 5o (EC50 between 3.2 μg/mL and 14.1 μg/mL) were more active than PCA (EC50 18.6 μg/mL) against Rhizoctonia solani. Especially conjugate 5c showed the higher fungicidal activity against Rhizoctonia solani which is 6.5-fold than PCA. And the results of the bioassay indicated that the fungicidal activity of conjugates was associated with their LogP, and the optimal LogP values of the more potent fungicidal activities within these conjugates ranged from 4.42 to 5.08. The systemic acquired resistance induced by PCA–SA ester conjugate 5c against rice sheath blight disease in rice seedlings was evaluated. The results revealed that PCA–SA ester conjugate 5c retained the resistance induction activity of SA against rice sheath blight. Electronic supplementary material The online version of this article (10.1186/s13065-018-0478-2) contains supplementary material, which is available to authorized users.

Salicylic acid (SA) (Fig. 2), also known as o-hydroxybenzoic acid which is one of the three isomers of hydroxybenzoic acid, is an important plant growth regulator playing a role in the hypersensitive reaction (HR) and acts as an endogenous signal responsible for inducing systemic acquired resistance in plants [22,23]. The plants treated with salicylic acid or its derivatives may be able to resist infection by various plant pathogens [24][25][26]. Hydroxybenzoate esters, which are widely used in medicine, foods and cosmetics, have been reported to have various biological activities, such as antimicrobial [27][28][29] antiviral [30,31], anti-inflammatory and nematicidal activities [32], among others. Accordingly, hydroxybenzoate esters with multiple bioactive chemical structures, have drawn wide attention in the biological and pharmacological fields.
In this research, considering the potential biological activity of phenazine-1-carboxylic derivatives and that there have been few published studies on the biological activity of phenazine-1-carboxylic phenolic esters, we designed and synthesized 16 novel phenolic ester derivatives of phenazine-1-carboxylic acid (Fig. 2) by a simple esterification reaction of PCA and three types of hydroxybenzoic acids. To enhance the lipophilic properties of the these conjugates, hydroxybenzoic acids were derivatized to its ester with the corresponding CH 3 (CH 2 )nOH. The synthetic route of conjugates 5a-5p is described in Fig. 3. All these conjugates were evaluated for their fungicidal activity against five phytopathogenic fungi in vitro. Furthermore, the systemic acquired resistance of the most active PCA-SA ester conjugate 5c against rice sheath blight disease was also investigated in rice plants.
The structures of all conjugates were characterized by 1 H NMR and high resolution mass spectroscopy (HRMS) analyses, and the representative conjugate 5d was confirmed by the X-ray crystallographic analysis. The molecular structure of 5d is shown in Fig. 4. The crystal data for 5d: triclinic, space group P2 1 /c, a

Fungicidal activities
All novel conjugates (5a-5p) were primarily screened in vitro against five phytopathogenic fungi, R. solani, A. solani, Fusarium oxysporum, Fusaium graminearum and P. oryzae, with PCA as a control. The results of the preliminary bioassay are shown in Table 1. We found that most of conjugates (5a-5p) showed low activities against A. solani, F. oxysporum, F. graminearum and P. oryzae Cavara at a concentration of 50 μg/mL, while most conjugates (5a-5p) exhibited high activity against R. solani at that rate. The inhibitory activity of 5c, 5e, 5i and 5m was 100%, higher than PCA at 86.2%. To more closely examine preliminary structure-activity relationships (SARs), the conjugates (5a-5p) were selected for assessment of EC 50 values against Rhizoctonia solani.
The recent study on fungicidal mechanism of PCA indicate that, PCA will promote cell produces poisonous hydroxyl radical and disrupt the normal homeostasis of redox in cells after entering cells through cell walls and cell membranes [19,33]. It means that a PCA analog with suitable polarity and hydrophobicity can pass through the cell membranes of pathogenic bacteria and fungi more easily and exhibit higher biological activity. As can be seen from Table 2, the fungicidal activities of conjugates were associated with their LogP values. Accordingly, we constructed a mathematical model that described the LogP of conjugates that might be expected to produce high or low levels of fungicidal activity. From Fig. 5, with increasing LogP values, the fungicidal activities of conjugates were also observed to increase. For instance, the LogP values of PCA-salicylic acid ester conjugates were ranked as follows: 5a < 5b < 5c, and the fungicidal  activity of conjugates also showed the same ranking. However, the conjugates that exceeded a certain level of LogP values (> 4.72) had decreased fungicidal activity. For instance, the LogP values of PCA-salicylic acid ester conjugates were ranked 5c < 5d < 5e, but the fungicidal activity of conjugates were ranked 5c > 5d > 5e. The same trends also applied to the PCA-3-hydroxybenzoic acid ester conjugates (5f-5j) and the PCA-p-hydroxybenzoic acid ester conjugates (5k-5p). Through the above analysis, we found that the LogP values of the more potent fungicidal activity within these three types of conjugates ranged from 4.42 to 5.08. Furthermore, conjugates where phenolic ester groups were substituted at different positions did not greatly affect their fungicidal activity.

Systemic acquired resistance
To evaluate the level of systemic acquired resistance induced by PCA-SA ester conjugates, the disease reduction of the most active PCA-SA ester conjugate 5c was investigated against rice sheath blight disease on rice seedlings following Makandar and others [34,35]. The results of the study indicated that inoculation with conidia of Rhizoctonia solani onto rice plants treated with SA and conjugate 5c resulted in fewer lesions per leaf sheath as well as reduced blighted leaf area as compared to control plants only receiving distilled water treatment (Fig. 6). Spray treatment with SA and PCA-SA ester conjugate 5c induced resistance to sheath blight disease in rice plants, significantly reducing rice sheath blight disease in rice plants. Compared with the treatments of PCA and water control, combined SA and conjugate 5c treatments had higher induction effects, at 31.0% and 57.0% respectively (Table 3). At present, there is extensive research on possible structure-activity relationship of SA and its derivatives for induction of systemic acquired resistance. Safari assessed the potential of some chemical inducers of systemic acquired resistance (SAR) to reduce Alternaria leaf spot disease on tomato in glasshouse trials [26]. The results indicated that, among the salicylate derivatives, the biochemical activators containing electron donating groups are more suitable for inducing disease resistance in tomato crop. Also the structure relationship of 47 mono-substituted and multi-substituted salicylate derivatives with respect to their effects on disease resistance to tobacco mosaic virus and pathogenesis-related protein (PR1) accumulation were evaluated [25]. In this study, using this characteristic of SA, we demonstrated  that PCA-SA ester conjugate 5c retained the resistance induction activity of SA against rice sheath blight and had higher induced resistance than SA. However, the relationship between the structures of PCA-SA ester conjugates described here and their induced activities needs further investigation, as well as the mode of action.

Chemicals and instruments
All chemicals and solvents were obtained from commercial suppliers and were used without further purification. The melting points were determined on a WRR melting point apparatus (Shanghai Jingke Industrial Co. Ltd., PR China) and were uncorrected. Thin-layer chromatography (TLC) was performed on silica gel 60 F254 (Qingdao Marine Chemical Ltd., P. R. China). Column chromatography (CC) purification was performed over silica gel (200-300 mesh, Qingdao Marine Chemical Ltd.). 1 H NMR spectrum were recorded in CDCl 3 solution on a Bruker 600 MHz spectrometer (Bruker Co., Switzerland), using tetramethylsilane (TMS) as an internal standard, and chemical shift values (δ) were given in parts per million (ppm). The following abbreviations were used to designate chemical shift multiplicities: s = singlet, d = doublet, t = triplet, q = quartet, m = multiple. MS data were obtained using a APEX IV Fourier-transform mass spectrometry (Bruker).

Synthesis of hydroxybenzoic acid esters
The compound 2-hydroxybenzoic acid (15 mmol) and its corresponding alcohol (30 mL) were added into a 50 mL round-bottom flask, and cooled at 0 °C. An aliquot of 2 mL of 98% H 2 SO 4 was slowly added. The reaction was stirred at reflux temperature for 12 h and monitored by thin-layer chromatography (TLC) until the 2-hydroxybenzoic acid was completely consumed. The mixture was evaporated under vacuum, neutralized with water and 5% NaHCO 3 aqueous solution, extracted by ether 3 times, dried over Na 2 SO 4 , concentrated in vacuum, and used in next step without purification. The compounds 3-hydroxybenzoic acid esters and p-hydroxybenzoic acid esters were also synthesized by this method.

Synthesis of phenazine-1-carbonyl chloride
Phenazine-1-carboxylic acid (10 mmol) and N,N-dimethylformamide (0.1 mmol) were added in 30 mL of dry CH 2 Cl 2 , and cooled at 0 °C. A solution of 15 mmol of oxalyl chloride in 20 mL of dry CH 2 Cl 2 was then slowly added. The reaction was stirred at reflux temperature for 12 h, then cooled to room temperature and evaporated under vacuum. The residue was dissolved in 10 mL of dry CH 2 Cl 2 and used in next step without purification.
General procedure for hydroxybenzoic acid ester conjugates of phenazine-1-carboxylic acid 5a-5p Phenazine-1-carbonyl chloride (10 mmol) dissolved in 10 mL of dry CH 2 Cl 2 was added dropwise to a solution of compound 2-hydroxybenzoic acid methyl ester (10 mmol), and triethylamine (12 mmol) as the attaching acid agent in CH 2 Cl 2 , The mixture was stirred at room temperature for 4 h until the reaction was complete (indicated by TLC), then quenched with water and 5% Na 2 CO 3 aqueous solution, dried over Na 2 SO 4 , filtered and concentrated in vacuum. The obtained crude extract was purified by recrystallizing from the solution of EtOAc-DCM (1:1) to give pure conjugate 5a. Conjugates 5b-5p were also synthesized by this method.

Biological assays
Compounds were screened for their in vitro fungicidal activity against Rhizoctonia solani, Fusaium graminearum, Altemaria solani, Fusarium oxysporum, Sclerotinia sclerotiorum and Pyricularia oryzae with the mycelium growth rate test. The method for testing the primary biological activity was performed aseptically with pure cultures. Synthesized compounds were dissolved in 100% acetone, and the solutions were diluted with aqueous 1% Tween 80 and were then added to sterile potato dextrose agar (PDA). The target final concentration of each compound was 50 μg/mL. The control blank assay was performed with 1 mL of sterile water. Mycelial plugs 6 mm in diameter were obtained with a cork borer and placed on the amended PDA. The culture plates were incubated at 28 °C. The diameter of the mycelia was measured after 72 h. Acetone in sterile aqueous 1% Tween 80 served as the negative control, whereas phenazine-1-carboxylic acid served as positive controls. Each sample was screened with three replicates, and each colony diameter of the three replicates was measured four times. All statistical analysis was performed using EXCEL 2010 software. The log dose-response curves allowed determination of the EC 50 for the bioassay using probit analysis. The 95% confidence limits for the range of EC 50 values were determined by the least-square regression analysis of the relative growth rate (% control) against the logarithm of the compound concentration. The relative inhibition rate of the circle mycelium compared to blank assay was calculated via the following equation: where CK is the extended diameter of the circle mycelium during the blank assay; and PT is the extended diameter of the circle mycelium during testing.

Plant materials and fungal growth condition
Seeds of rice (Feng liang you xiang No. 1), with high rates of germination, were grown in plastic pots of 20 cm diameter and kept in a greenhouse under a temperature of 26-28 °C, with 10 plant per pot. After 4 weeks Relative inhibition rate (%) the four-leaf stage plants were used in the experiments. Rhizoctonia solani was cultured for 4 days at 28 °C on potato dextrose agar (PDA), under aseptic conditions. Spore concentration was adjusted with sterile distilled water to 10 5 spores/mL.

Chemical treatment of plants
Chemical treatments of plants were carried out as described by Makandar and others [34,35]. Briefly, a stock solution of 10 mmol/L for testing conjugate 5c (highest fungicidal activity against Rhizoctonia solani) was prepared in water and diluted to a final concentration of 200 μmol/L. Rice plants at the four-leaf of the similar size were sprayed with a concentration of 200 μmol/L of test conjugate 5c, PCA and of salicylic acid (SA). A blank water control was also applied under the same conditions. There were four treatments as follows: (1) PCA, (2) SA, (3) conjugate 5c, and water-treated control. Each treatment consisted of three pots each containing 10 rice seedlings, and were arranged in a completely randomized design and replicated four times. In all treatments, spraying was done 24 h prior to inoculation.

Fungal inoculation and disease rating
Plants were treated with chemicals and 24 h later, point inoculations of rice leaf sheaths were done with needle injection of 10 μL of the 10 5 spores/mL suspension at the four-leaf stage of seedlings of rice. For each replication of each treatment, 30 leaf sheaths were inoculated. The inoculated plants were covered with black plastic bags and kept in a growth room maintained at 90% relative humidity near 90% at 26-28 °C for 24 h. Plants were evaluated for rice sheath blight disease as percent leaf sheath infected with Rhizoctonia solani at 14 days after inoculation. All statistical analyses were performed using EXCEL 2010 software. The disease reduction was calculated as follows: where CK is the percent disease in inoculated plants treated with water while PT is the disease rating for inducer treatments.