Effect of altitude and solvent on Psidium guajava Linn. leaves extracts: phytochemical analysis, antioxidant, cytotoxicity and antimicrobial activity against food spoilage microbes

Table 2 PCR conditions of 16 s and 18 s rRNA

Gene	Initial denaturation	Denaturation	Annealing	Extension	Final extension	Hold
16s rRNA	95 °C	95 °C	54 °C	72 °C	72 °C	4 °C
	2 min	30 s	30 s	160 s	5 min
			29 cycles
18s rRNA	95 °C	95 °C	50 °C	72 °C	72 °C	4 °C
	2 min	30 s	30 s	70 s	5 min
			29 cycles

ISSN: 2661-801X