Skip to main content

Table 5 Penalty points of greenness assessment of the chromatographic suggested methods by Eco-Scale

From: Green analytical chemistry and experimental design: a combined approach for the analysis of zonisamide

Reagents/Instruments

Proposed TLC method

Proposed HPLC method

*Official HPLC method [9]

Ethanol

 

4

 

Methanol

6

 

6

Phosphoric acid

  

2

Chloroform

4

  

Acetic acid

2

  

acetonitrile

  

4

TLC

1

  

HPLC

 

1

1

Occupational hazard

0

0

0

Waste

3

4

6

Total penalty points

Æ© 16

Æ© 9

Æ© 19

Analytical Eco-Scale total score

84

91

81

  1. *Official HPLC method using C18 column, acetonitrile: methanol: 1.36 g/L phosphate buffer (1:1:8 by volume) as a mobile phase and UV detection at 240 nm [9]
Back to article page

BMC Chemistry

ISSN: 2661-801X

Contact us