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Table 2 Assay and validation parameters for the determination of ZNS by the proposed methods

From: Green analytical chemistry and experimental design: a combined approach for the analysis of zonisamide

Parameter

TLC

HPLC

Range

2–10 µg/band

0.5-10 µg/mL

Slopea

− 83.851*

2094.5**

14,327

Intercept

5987.4

− 300.15

Correlation coefficient (r)

0.9997

1

Accuracy

99.97 ± 1.22

100.15 ± 0.52

Repeatability b

1.04

0.77

Intermediate precision c

0.94

0.88

Robustness d

1.28

1.21

LOD

0.63 µg/band

0.07 µg/mL

LOQ

1.90 µg/band

0.21 µg/mL

SE of Intercept

46.00

251.73

SE of slope

15.21

41.55

  1. aSlope * and ** are the coefficients of X2 and X, respectively. Following a polynomial regression y = ax2 + bx + c Where, A is the peak area, x is the concentration of ZNS (μg/band), a and b are coefficients * and **, respectively and c is the intercept
  2. bRSD (%) of three concentrations of ZNS (3, 5 and 7 µg/band for TLC) and (4, 6 and 8 µg/mL for HPLC) analyzed intra-daily in triplicate
  3. cRSD (%) of three concentrations of ZNS (3, 5 and 7 µg/band for TLC) and (4, 6 and 8 µg/mL for HPLC) analyzed inter-daily on three successive days
  4. dRSD% of ZNS at three different ratio mobile phases of ethanol: H2O (30:70 ± 2 mL of H2O) for HPLC and chloroform: methanol: acetic acid (8: 1.5: 0.5) ± 0.5 mL of methanol) for TLC
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BMC Chemistry

ISSN: 2661-801X

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