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Table 1 Parameters required for system suitability tests of TLC–densitometric and HPLC methods

From: Green analytical chemistry and experimental design: a combined approach for the analysis of zonisamide

Method

TLC

HPLC

Reference values [12]

Parameter

Zonisamide

Degradation product

Zonisamide

Degradation product

 

tR (min)

–

–

2.806

1.553

–

Rf

0.76

0.11

–

–

–

Resolution (Rs)

14.38

6

Rs > 2

Tailing factor (T)

1

0.9

1

0.83

≈1

Capacity factor (K)

0.31

9

4.61

2.11

0–10

Selectivity factor (α)

7.6

2.18

α > 1

Column efficiency (N)

–

–

3831.26

866.41

Increase with efficiency of the separation

Height equivalent to theoretical plates(HETP) (mm)

–

–

0.03

0.12

The smaller the value the higher the column efficiency