Method | TLC | HPLC | Reference values [12] | ||
---|---|---|---|---|---|
Parameter | Zonisamide | Degradation product | Zonisamide | Degradation product | Â |
tR (min) | – | – | 2.806 | 1.553 | – |
Rf | 0.76 | 0.11 | – | – | – |
Resolution (Rs) | 14.38 | 6 | Rs > 2 | ||
Tailing factor (T) | 1 | 0.9 | 1 | 0.83 | ≈1 |
Capacity factor (K) | 0.31 | 9 | 4.61 | 2.11 | 0–10 |
Selectivity factor (α) | 7.6 | 2.18 | α > 1 | ||
Column efficiency (N) | – | – | 3831.26 | 866.41 | Increase with efficiency of the separation |
Height equivalent to theoretical plates(HETP) (mm) | – | – | 0.03 | 0.12 | The smaller the value the higher the column efficiency |