Insights of different analytical approaches for estimation of budesonide as COVID-19 replication inhibitor in its novel combinations: green assessment with AGREE and GAPI approaches

Table 1 Optimization of chromatographic performance for simultaneous separation of BUD epimers and AZL by HPLC technique

Parameter		N			(D_m)			(α)		(Rs)
Parameter		BUD_{(Epimer B)}	BUD_{(Epimer A)}	AZL	BUD_{(Epimer B)}	BUD_{(Epimer A)}	AZL	BUD_(B)/AZL	BUD_(B)/BUD_(A)	BUD_(B)/AZL	BUD_(B)/BUD_(A)
Ratio of organic modifier A/B*	50:50	2204	2389	449	4.28	4.59	0.59	7.80	1.07	8.98	0.58
	45:55	3702	3598	1010	6.39	6.90	1.31	5.25	1.08	11.60	0.86
	42:58	4133	3995	1038	8.75	9.56	1.68	5.69	1.09	13.37	1.07
	40:60	4460	4202	1032	9.71	10.64	2.09	5.08	1.10	13.16	1.17
	30:70	3734	4101	936	15.97	17.49	3.00	5.83	1.09	14.68	1.13
pH	3.0	4208	3946	995	9.40	10.28	1.68	6.12	1.09	13.81	1.10
	3.5	4460	4202	1032	9.71	10.64	2.09	5.08	1.10	13.16	1.17
	4.0	4383	4154	929	9.34	10.27	2.36	4.35	1.10	11.78	1.20
	5.0	4416	4141	731	8.99	9.83	3.45	2.85	1.09	8.15	1.13
	6.0	4394	4119	775	9.35	10.23	5.30	1.93	1.09	5.22	1.12
Molar strength of buffer	0.02 M	4139	3879	1681	11.53	12.19	4.47	2.73	1.06	11.44	1.38
	0.03 M	4102	3954	1161	11.06	11.99	3.40	3.52	1.08	11.47	1.32
	0.04 M	4460	4202	1032	9.71	10.64	2.09	5.08	1.10	13.16	1.17
	0.05 M	4098	3774	436	8.93	9.88	0.97	10.2	1.11	14.87	0.95

Where: A/B*, A is acetonitrile and B is phosphate buffer (0.04 M)
(N) is number of theoretical plates = 5.45 (t_R/W_h/2)²
(D_m) is mass distribution ratio = t_R − t_m/t_m
(α) is selective factor = D_m2/D_m1
(Rs) is resolution = 2Δt_R/W₁ + W₂
t_R is the retention time of the substance measured from the point of injection while tm is the retention time of a non-retained marker
W_h/2 is the peak width at the half height
W₁ and W₂ are the width of the peaks of the two components at their bases

ISSN: 2661-801X