Table 9 Assay results for the determination of the ASP, ATR and RAM drugs in synthetic mixtures in ratios of 10:4:1 (w/w) by the proposed HPLC method
Compound | Proposed method | Comparison method [9] | |
|---|---|---|---|
Amount taken, (µg/mL) | % Found, % | % Found, % | |
ASP | 100 | 99.14 | 101.02 |
140 | 101.10 | 100.45 | |
200 | 99.71 | 100.58 | |
Mean ± SD | 99.98 ± 1.01 | 100.35 ± 0.73 | |
t-test | 1.15 (2.78) | ||
F- test | 11.39 (19.00) | ||
ATR | 40 | 99.33 | 99.67 |
56 | 100.84 | 100.28 | |
80 | 99.97 | 99.78 | |
Mean ± SD | 100.05 ± 0.76 | 99.91 ± 0.33 | |
t-test | 0.29 (2.78) | ||
F-test | 5.43 (19.00) | ||
RAM | 10 | 101.57 | 100.03 |
14 | 98.59 | 98.56 | |
20 | 100.31 | 101.46 | |
Mean ± SD | 100.16 ± 1.50 | 100.017 ± 1.45 | |
t-test | 0.12 (2.78) | ||
F-test | 1.06 (19.00) | ||