Parameter | Number of theoretical plates (N) | Resolution, RS | Selectivity, α | |||||
---|---|---|---|---|---|---|---|---|
ASP | ATR | RAM | ASP/ATR | ATR/RAM | ASP/ATR | ATR/RAM | ||
% Concentration n- propanol (v/v) | ||||||||
6 | 770 | 1271 | 746 | 2.9 | 4.2 | 3.5 | 2.78 | |
8 | 510 | 931 | 1005 | 2.7 | 4.7 | 3.7 | 2.9 | |
10 | 504 | 971 | 1317 | 2.7 | 4.6 | 3.1 | 2.7 | |
12 | 465 | 892 | 1336 | 1.9 | 4.42 | 3.6 | 3.4 | |
14 | 423 | 743 | 1402 | 3.5 | 3.6 | 16 | 2.7 | |
15 | 567 | 1112 | 363 | 5.1 | 2.7 | 18 | 2.2 | |
Concentration of SDS, M | ||||||||
0.05 | 467 | 1045 | 395 | 4.6 | 8 | 3 | 3.2 | |
0.08 | 970 | 1766 | 1172 | 4.36 | 7.2 | 3.4 | 3.2 | |
0.1 | 659 | 1229 | 1491 | 3 | 5 | 3.3 | 3.3 | |
0.12 | 515 | 869 | 1485 | 2 | 4.44 | 4 | 3.5 | |
0.15 | 480 | 859 | 1158 | 1.7 | 3.6 | 3.75 | 3.3 | |
0.18 | 414 | 658 | 1143 | 1.5 | 2.2 | 6 | 3.5 | |
pH of the medium | ||||||||
2.5 | 515 | 971 | 1485 | 3.2 | 5 | 3 | 2.66 | |
3 | 651 | 606 | 1173 | 2.2 | 5.87 | 3.75 | 4.1 | |
4 | 308 | 1207 | 207 | 0.32 | 6.35 | 1.17 | 26.76 | |
5 | Overlapped | 343 | ||||||
6 | Overlapped peaks | |||||||
Flow rate, mL/min. | ||||||||
0.8 | 417 | 1094 | forked | 2.6 | 3.36 | 4.42 | 2.64 | |
1 | 469 | 987 | 1380 | 2.38 | 4.4 | 4.16 | 3.12 | |
1.2 | 458 | 974 | 1376 | 2.8 | 4.16 | 4.24 | 3.76 | |
1.5 | 515 | 871 | 1485 | 2.54 | 4.2 | 4 | 3.37 | |
2 | 473 | 964 | 1210 | 2.26 | 4.34 | 5.5 | 3.36 |